Table 3.

The effects of the interval between experiments and the total number of experiments on E2 and GnRH levels

Experimental conditionsMean E2 (pg/ml)Mean GnRH (pg/ml)
Intervals between two probe insertions
    0–21 d from last experiment (n = 17)127 ± 58a4.48 ± 1.46b
    22–50 d from last experiment (n = 14)152 ± 59a3.26 ± 0.41b
Total number of experiments
    1–5 experiments conducted (n = 9)118 ± 58c3.75 ± 1.28d
    5–10 experiments conducted (n = 8)179 ± 136c2.24 ± 0.36d
    10–15 experiments conducted (n = 14)139 ± 59c4.54 ± 1.38d
  • Retrospective analyses indicate that, reactive glia are not a likely a source of E2 release in the S-ME, as neither time between two microdialysis experiments nor the number of experiments were correlated with mean E2 and GnRH levels. The same letter indicates no difference between conditions by Student's t test (for the intervals between two probe insertions) or 1-way ANOVA (for the total number of experiments).

  • a: Mean E2 (p = 0.7658);

  • b: Mean GnRH (p = 0.4575);

  • c: Mean E2 (p = 0.8874);

  • d: Mean GnRH (p = 0.5967). Note that the total number of experiments can reach up to 15 times per animal, as the same animals were also co-assigned to two additional unrelated microdialysis sampling projects.