Construct name | Forward primer | Reverse primer |
---|---|---|
EGFP- and mRFP-Rab5a | GATCTC GAGCTC C ATGGCAAACCGAGGCGGAG | ATTACA CCCGGG GATGGCACAAAGGCCAAGACAGG |
EGFP-and mRFP-Rab5c | TCAGAT CTCGAG C ATGGCCAGTAGAGGAACAAGCG | ACTGCAGAATTC GAAGTGACTGTAGAGCGACGGTG |
CA-Rab5c (Q80L) | CTGGGACACCGCAGGGCTGGAGAGGTACCACAG | CTGTGGTACCTCTCCAGCCCTGCGGTGTCCCAG |
EGFP- and mRFP-Rab4 | GATCTC GAGCTC G TCTGAGACATACGACTTCCTGTTCA AG | ATTACA CCCGGG ATGAGCAAGCTTCCAGGGCAAG |
CA-Rab4 (Q67L) | CTGGGATACGGCAGGACTGGAGAGATTCAGATCGG | CCGATCTGAATCTCTCCAGTCCTGCCGTATCCCAG |
DN-Rab4 (N121I) | CTTGTGTGGAATCAAAAAAGATTTGGATGCTGATCGAGAGGTG | CACCTCTCGATCAGCATCCAAATCTTTTTTGATTCCACACAAG |
EGFP- and mRFP-Rab7a | GATCTC GAGCTC C ACG AG C AGGAAGAAAGTGCTGCT GAAGGTC | GTGGAT CCCGGG GTTAGTGAAGGACTCCGGCTTCG |
CA-Rab7 (Q67L) | GATATGGGACACAGCTGGGCTGGAGAGGTTCCAATCG | TGGAACCTCTCCAGCCCAGCTGTGTCCCATATCTGC |
DN-Rab7 (T22N) | GGGTTGGCAAAAACTCTCTCATGAACCAGTATGTG | CACATACTGGTTCATGAGAGAGTTTTTGCCAACCCC |
EGFP- and mRFPRab11a | CACTGA GAGCTC G ATGGGTACCAGGGACGACG | GTGGAT CCCGGG AAAGAGTCCAGGGCAGGACG |
EGFP- and mRFP-Rab11b | TCAGAT CTCGAG C GGAGCCAGAGATGACGAATACG | ACTGCAGAATTC CTCAGACTACGTCAGGTCC |
The extra 5′ sequences that contain the restriction site are in italics. The codons that were modified by site-directed mutagenesis are underlined.