Table 1.

List of the primers used to generate the Xenopus Rab constructs

Construct nameForward primerReverse primer
EGFP- and mRFP-Rab5aGATCTC GAGCTC C ATGGCAAACCGAGGCGGAGATTACA CCCGGG GATGGCACAAAGGCCAAGACAGG
EGFP-and mRFP-Rab5cTCAGAT CTCGAG C ATGGCCAGTAGAGGAACAAGCGACTGCAGAATTC GAAGTGACTGTAGAGCGACGGTG
CA-Rab5c (Q80L)CTGGGACACCGCAGGGCTGGAGAGGTACCACAGCTGTGGTACCTCTCCAGCCCTGCGGTGTCCCAG
EGFP- and mRFP-Rab4GATCTC GAGCTC G TCTGAGACATACGACTTCCTGTTCA AGATTACA CCCGGG ATGAGCAAGCTTCCAGGGCAAG
CA-Rab4 (Q67L)CTGGGATACGGCAGGACTGGAGAGATTCAGATCGGCCGATCTGAATCTCTCCAGTCCTGCCGTATCCCAG
DN-Rab4 (N121I)CTTGTGTGGAATCAAAAAAGATTTGGATGCTGATCGAGAGGTGCACCTCTCGATCAGCATCCAAATCTTTTTTGATTCCACACAAG
EGFP- and mRFP-Rab7aGATCTC GAGCTC C ACG AG C AGGAAGAAAGTGCTGCT GAAGGTCGTGGAT CCCGGG GTTAGTGAAGGACTCCGGCTTCG
CA-Rab7 (Q67L)GATATGGGACACAGCTGGGCTGGAGAGGTTCCAATCGTGGAACCTCTCCAGCCCAGCTGTGTCCCATATCTGC
DN-Rab7 (T22N)GGGTTGGCAAAAACTCTCTCATGAACCAGTATGTGCACATACTGGTTCATGAGAGAGTTTTTGCCAACCCC
EGFP- and mRFPRab11aCACTGA GAGCTC G ATGGGTACCAGGGACGACGGTGGAT CCCGGG AAAGAGTCCAGGGCAGGACG
EGFP- and mRFP-Rab11bTCAGAT CTCGAG C GGAGCCAGAGATGACGAATACGACTGCAGAATTC CTCAGACTACGTCAGGTCC

  • The extra 5′ sequences that contain the restriction site are in italics. The codons that were modified by site-directed mutagenesis are underlined.