Distribution of synapses over the dendritic arbor of cholinergic interneurons
| Properties examined | Proximal (0–19%) | Distal (20–79%) | Most distal (80–100%) |
|---|---|---|---|
| Number of dendritic fragments analyzed | 17 | 38 | 9 |
| Average distance from soma (±SEM) (μm) | 59.96 (±12.19) | 276.53 (±34.22) | 558.22 (±17.00) |
| Average distance from soma as a percentage of total distance (±SEM) | 9.31 (±1.90) | 43.64 (±5.40) | 85.48 (±2.17) |
| Ultrathin sections analyzed (total) | 730 | 1568 | 379 |
| Average number of ultrathin sections analyzed in serial section (±SEM) | 42.94 (±7.02) | 41.26 (±6.26) | 42.11 (±10.13) |
| Total number of terminals forming asymmetric synapses | 24 | 58 | 19 |
| In VGluT1-labeled tissue | 14 | 40 | 6 |
| In VGlut2-labeled tissue | 10 | 18 | 13 |
| Total number of VGlut1-positive terminals forming synapses | 1 | 1 | 0 |
| Total number of VGlut2-positive terminals forming synapses | 2 | 10 | 2 |
| Total number of terminals forming symmetric synapses | 55 | 103 | 29 |
The distribution of terminals forming synapses on dendrites at varying distances from the soma was analyzed for the three neurons examined.