Table 4.

Typical ion concentrations used in different in vitro models of SPW HFOs

Article	Species	Age	Slice width (μm)	Slice recovery/recording	KCl	KH₂PO₄	NaH₂PO₄	MgSO₄	MgCl₂	CaCl₂
Viereckel et al., 2013	C57 mice	4–8 weeks	450	Interface/interface	3		1.25	1.8		1.6
Maier et al., 2003	C57 mice	4–12 weeks	450	Interface/interface	3		1.25	1.8		1.6
Holderith et al., 2011	CD1 mice	14–26 days	350–400	Interface/submerged	2.5		1.25		2	2
Behrens et al., 2005	W rat	5–8 weeks	400–12°	Interface/?	3		1.25	1.8		1.6
Sun et al., 2012	C57 mice	3–5 weeks	400–12.7°	Submerged/submerged	3.5		1.24	1.3		2
Norimoto et al., 2012	C57 mice		400–12.7°	Submerged/submerged	3.5	1.24		1.2		2
Kubota et al., 2003	SD rat	4 weeks	350	Interface/interface	3	1.25		1		3
Colgin et al., 2004	SD rat	4–5 weeks	350	Interface/interface	3	1.25		3		1
Foffani et al., 2007	SD rat	6–7 weeks	350	Submerged/interface	4		1.2		1	1
Papatheodoropoulos, 2008	W rat	4–6 weeks	500–550	Interface/interface	4		1.25	2		2
Kanak et al., 2013	C57 mice	4–21 months	400	Interface/interface	3		1.4	1.3		2.5
Hájos et al., 2009	W rat/CD1 mice	14–20 days	400–450	Interface and submerged/same	3	1.25		1		3
Ellender et al., 2010	W rat	14–24 days	400	Interface/submerged	3	1.25		1		3
Simeone et al., 2013	C3HeB/FeJ mice	30–45 days	350	?/submerged	3	X	1.25	2.5	X	2.4
Maier et al., 2009	C57 mice	4–8 weeks	400	Submerged/submerged	2.5		1		1.3	2.5
Maier et al., 2012	C57 mice	4–8 weeks	400	Interface/submerged	2.5		1	1.3		2.5
Wu et al., 2009	C57BL/6 mice	21 days to 4 months	500	Submerged/submerged	3.5		1.25	1.3		2

NaCl concentration ranged from 124 to 129 mm. Glucose concentration was 10 mm in all cases. NaHCO₃ concentration ranged from 21 to 26 mm.