Table 1.

5′ RACE and 3′ RACE was performed using SMART RACE cDNA Amplification Kit (Clontech)a

NameSequence
NKCC1 Ex2 (3′RACE) F1 primer5′-TGAGGATGGCTTTGCAAATGGGGAAG-3′
NKCC1 Ex2 (3′RACE) F2 primer5′-CTCCAACCAGAGATGCTGTGGTCACG-3′
NKCC1 Ex6, 7 (3′RACE) F1 primer5′-GCTGGAATGGAGTGGGAAGCAAAAGC-3′
NKCC1 Ex6, 7 (3′RACE) F2 primer5′-CCCACTGGAGAGCAAGAAGCCAAAAGG-3′
NKCC1 Ex14, 15 (3′RACE) F1 primer5′-TCGTCATTAACTGGTGGGCTGCATTGCT-3′
NKCC1 Ex14, 15 (3′RACE) F2 primer5′-TTGGGGATCCTCTACACAAGCCCTGA-3′
NKCC1 Ex2 (5′RACE) R1 primer5′-CACCCTTGATCCAGCCAAACTTCACGA-3′
NKCC1 Ex2 (5′RACE) R2 primer5′-CTTCCCCATTTGCAAAGCCATCCTCA-3′
NKCC1 Ex6, 7 (5′RACE) R1 primer5′-CCCTTTTGGCTTCTTGCTCTCCAGTG-3′
NKCC1 Ex6, 7 (5′RACE) R2 primer5′-CTGAGCTTTTGCTTCCCACTCCATTCCA-3′
NKCC1 Ex13, 14 (5′RACE) R1 primer5′-GCAATGCAGCCCACCAGTTAATGACGA-3′
NKCC1 Ex13, 14 (5′RACE) R2 primer5′-GGACGCCATCCTGGAGATTTTGCAAGTG-3′
  • aWe used human adult brain reference RNA (Ambion) and human 20- to 33-week fetal brain poly A+ RNA (Clontech), and these RNAs were reverse-transcribed to cDNA by MMLV reverse transcriptase (Clontech). Enzyme and buffer for RACE PCR reactions were used from Advantage-GC 2 PCR Kit (Clontech). Antisense primers for 5′ RACE and sense primers for 3′ RACE, which we designed, are shown in Table 1.