Table 1.

Overview of gait parameters measured in Noldus Catwalk

ParameterDefinitionPawControlCKOp
Run duration (s)Time for traveling down the walkway2.93 ± 0.232.59 ± 0.250.33
Swing speed (cm/s)Speed of movementRF45.65 ± 1.7651.63 ± 3.720.20
RH56.84 ± 1.4861.95 ± 2.870.16
LF45.46 ± 1.8851.00 ± 3.750.24
LH58.96 ± 1.8661.40 ± 3.090.53
Normalized swing speed (cm)Swing speed × run durationRF130.6 ± 5.72122.4 ± 3.760.23
RH166.2 ± 12.70152.0 ± 8.210.34
LF129.4 ± 4.21120.7 ± 4.100.16
LH171.0 ± 11.29150.3 ± 8.200.14
Stride lengthb (cm)Distance between successive steps of the same pawRF5.50 ± 0.296.28 ± 0.240.048*
RH5.44 ± 0.275.96 ± 0.2010.13
LF5.54 ± 0.296.22 ± 0.210.062
LH5.35 ± 0.265.89 ± 0.270.17
Stand (s)Time of paw contact with the walkwayRF0.19 ± 0.0130.18 ± 0.0140.49
RH0.23 ± 0.0170.22 ± 0.0220.72
LF0.19 ± 0.0120.18 ± 0.0140.55
LH0.23 ± 0.0160.21 ± 0.0200.48
Base of supportc (cm)Width between either the front of hind pawsFront1.32 ± 0.0421.17 ± 0.0410.020*
Hind2.63 ± 0.0642.64 ± 0.0540.91
Print positiond (cm)Distance between hind paw to previous position of ipsilateral front pawRF-RH0.92 ± 0.120.45 ± 0.130.013*
LF-LH0.94 ± 0.120.47 ± 0.130.017*
Duty cycle (%)Stand as a percentage of step cycleRF57.60 ± 1.0055.51 ± 1.030.17
RH67.27 ± 1.6865.97 ± 1.610.58
LF57.70 ± 1.0555.29 ± 1.250.17
LH68.96 ± 1.5664.76 ± 1.390.056
Regularity index (%)Normal step sequence pattern relative to paw placements93.86 ± 1.4591.57 ± 2.040.39
  • aThe same cohort of mice dMHbCKO and matched control mice was used for all of the experiments shown (n = 11 control and n = 14 dMHbCKO mice). RF, Right front paw; RH, right hind paw; LF, left front paw; LH, left hind paw.

  • bThe distance between successive steps of the right front paw was longer in the dMHbCKO mice compared with the control mice.

  • cForelimb BOS was shorter for the dMHbCKO mice compared with the control mice.

  • dPrint position was shorter in the dMHbCKO mice compared with the control mice.

  • *p < 0.05.