Groupa | Time point (wk)b | Animal no. | Experimentc | Corresponding figure |
---|---|---|---|---|
Sham (n = 23) | 9 | 8 | Behavior (Catwalk) | Fig. 1 |
3 | 5 | ELISA (SCS) | Fig. 5 | |
3 | 3 | Western blot | Fig. 7A–C | |
3 | 7 | ELISA (SEC) | Fig. 11 | |
PBS (n = 18) | 9 | 8 | Behavior, histology | Figs. 1, Figs. 9, Figs. 10 |
3 | 3 | Western blot | Fig. 7A–C | |
3 | 7 | ELISA (SEC) | Fig. 11 | |
SCI (n = 5) | 3 | 5 | ELISA (SCS) | Fig. 5 |
TP (n = 32) | 10 d | 4 | Histology | Figs. 2, Figs. 4 |
3 | 8 | Histology | Figs. 2, Figs. 3, | |
5 | 5 | Histology | Fig. 2 | |
9 | 8 | Behavior, histology | Figs. 1, Figs. 2, Figs. 3, Figs. 9, Figs. 10 | |
3 | 7 | ELISA (SEC) | Fig. 11 | |
TMT (n = 15) | 9 | 8 | Behavior, histology | Figs. 1, Figs. 9, Figs. 10 |
3 | 7 | ELISA (SEC) | Fig. 11 | |
SCI with TMT (n = 5) | 3 | 5 | ELISA (SCS) | Fig. 5 |
TP + TMT (n = 32) | 10 d | 4 | Histology | Figs. 2, Figs. 4, |
3 | 8 | Histology | Figs. 2, Figs. 3, | |
5 | 5 | Histology | Fig. 2 | |
9 | 8 | Behavior, histology | Figs. 1, Figs. 2, Figs. 3, Figs. 9, Figs. 10 | |
3 | 7 | ELISA (SEC) | Fig. 11 | |
TP with IgG (n = 6) | 5 | 6 | Histology | Figs. 6, Figs. 7D–H |
TP with αBDNF (n = 6) | 5 | 6 | Histology | Figs. 6 |
TP with αNT-3 (n = 6) | 5 | 6 | Histology | Figs. 6 |
TP with αIGF-1 (n = 6) | 5 | 6 | Histology | Figs. 6, Figs. 7D–H |
TP + TMT with IgG (n = 9) | 5 | 6 | Histology | Figs. 6, Figs. 7D–H |
3 | 3 | Western blot | Fig. 7A–C | |
TP + TMT with αBDNF (n = 6) | 5 | 6 | Histology | Fig. 6 |
TP + TMT with αNT-3 (n = 6) | 5 | 6 | Histology | Fig. 6 |
TP + TMT with αIGF-1 (n = 9) | 5 | 6 | Histology | Figs. 6, Figs. 7D–H |
3 | 3 | Western blot | Fig. 7A–C | |
Total | — | 184 | — | — |
↵aGroup designations: Sham, sham-operated (laminectomy only) animals; PBS, injured animals with PBS injection (control for NSC transplantation); SCI, animals with injury alone; TP, animals that received NSC TP at 1 week after injury; TMT, animals with TMT alone (PBS injection without TP); SCI with TMT, injured animals with TMT alone; TP + TMT, animals with a combination of TP + TMT. See the Materials and Methods for detailed group designations. Numbers in parentheses indicate the subtotal number of animals in each group.
↵bTime point refers to the duration elapsed after injury.
↵cELISA (SCS), ELISA experiment measuring levels of BDNF, NT-3, and IGF-1 in serum, CSF, and spinal cord parenchyma (epicenter); ELISA (SEC) ELISA experiment measuring levels of BDNF, NT-3, and GDNF in the spinal cord tissue at the epicenter and caudal regions.