Table 1.

Number of animals assigned for separate experimental groups and for separate end time points in different experiments

GroupaTime point (wk)bAnimal no.ExperimentcCorresponding figure
Sham (n = 23)98Behavior (Catwalk)Fig. 1
35ELISA (SCS)Fig. 5
33Western blotFig. 7A–C
37ELISA (SEC)Fig. 11
PBS (n = 18)98Behavior, histologyFigs. 1, Figs. 9, Figs. 10
33Western blotFig. 7A–C
37ELISA (SEC)Fig. 11
SCI (n = 5)35ELISA (SCS)Fig. 5
TP (n = 32)10 d4HistologyFigs. 2, Figs. 4
38HistologyFigs. 2, Figs. 3,
55HistologyFig. 2
98Behavior, histologyFigs. 1, Figs. 2, Figs. 3, Figs. 9, Figs. 10
37ELISA (SEC)Fig. 11
TMT (n = 15)98Behavior, histologyFigs. 1, Figs. 9, Figs. 10
37ELISA (SEC)Fig. 11
SCI with TMT (n = 5)35ELISA (SCS)Fig. 5
TP + TMT (n = 32)10 d4HistologyFigs. 2, Figs. 4,
38HistologyFigs. 2, Figs. 3,
55HistologyFig. 2
98Behavior, histologyFigs. 1, Figs. 2, Figs. 3, Figs. 9, Figs. 10
37ELISA (SEC)Fig. 11
TP with IgG (n = 6)56HistologyFigs. 6, Figs. 7D–H
TP with αBDNF (n = 6)56HistologyFigs. 6
TP with αNT-3 (n = 6)56HistologyFigs. 6
TP with αIGF-1 (n = 6)56HistologyFigs. 6, Figs. 7D–H
TP + TMT with IgG (n = 9)56HistologyFigs. 6, Figs. 7D–H
33Western blotFig. 7A–C
TP + TMT with αBDNF (n = 6)56HistologyFig. 6
TP + TMT with αNT-3 (n = 6)56HistologyFig. 6
TP + TMT with αIGF-1 (n = 9)56HistologyFigs. 6, Figs. 7D–H
33Western blotFig. 7A–C
Total184
  • aGroup designations: Sham, sham-operated (laminectomy only) animals; PBS, injured animals with PBS injection (control for NSC transplantation); SCI, animals with injury alone; TP, animals that received NSC TP at 1 week after injury; TMT, animals with TMT alone (PBS injection without TP); SCI with TMT, injured animals with TMT alone; TP + TMT, animals with a combination of TP + TMT. See the Materials and Methods for detailed group designations. Numbers in parentheses indicate the subtotal number of animals in each group.

  • bTime point refers to the duration elapsed after injury.

  • cELISA (SCS), ELISA experiment measuring levels of BDNF, NT-3, and IGF-1 in serum, CSF, and spinal cord parenchyma (epicenter); ELISA (SEC) ELISA experiment measuring levels of BDNF, NT-3, and GDNF in the spinal cord tissue at the epicenter and caudal regions.