Mouse primers for qPCR
| Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|
| Claudin-1 | ACTCCTTGCTGAATCTGAACAGT | GGACACAAAGATTGCGATCAG |
| Claudin-3 | TGGGAGGGCCTGTGGAT | CGTACATTTTGCACTGCATCTGA |
| Claudin-5 | CTGCCTTCCTGGACCACAA | TCCACAGCCCCTTCCAAGT |
| COX-2 | CAGGTCATTGGTGGAGAGGTGTA | GGATGTGAGGAGGGTAGATCATCT |
| ET-1 | GTGTCTACTTCTGCCACCTG | CACTGACATCTAACTGCCTGG |
| GAPDH | TGTGTCCGTCGTGGATCTGA | CCTGCTTCACCACCTTCTTGA |
| GLUT1 | GGTGTGCAGCAGCCTGTGT | CACAGTGAAGGCCGTGTTGA |
| eNOS | TCTGCGGCGATGTCACTATG | CATGCCGCCCTCTGTTG |
| iNOS | GGCAGCCTGTGAGACCTTTG | TGCATTGGAAGTGAAGCGTTT |
| Occludin | TCAAACCGAATCATTATGCACCA | AGATGGCAATGCACATCACAA |
| ZO-1 | GAGCTACGCTTGCCACACTGT | TCGGATCTCCAGGAAGACACTT |
| ZO-2 | CTTCTCACATTCAAAGTGGCTTC | CTACCTGCTAGAAATCCCCAAA |