Table 4.

Quantification of TDP-43 distribution in polysome fractions

TDP-43WT (absolute value; ratio to input)TDP-43G298S (absolute value; ratio to input)
Input1.04 ± 0.021.15 ± 0.12
RNP1.05 ± 0.02; 1.01 ± 0.0011.14 ± 0.12; 0.99 ± 0.003
40S/60S0.97 ± 0.06; 0.93 ± 0.041.05 ± 0.05; 0.93 ± 0.07
80S (monosome)0.83 ± 0.05; 0.80 ± 0.030.88 ± 0.03; 0.78 ± 0.05
2 ribosomes0.90 ± 0.04; 0.87 ± 0.020.85 ± 0.01; 0.75 ± 0.07
3 ribosomes0.85 ± 0.04; 0.82 ± 0.030.85 ± 0.03; 0.76 ± 0.08
4 ribosomes0.84 ± 0.05; 0.80 ± 0.030.84 ± 0.08; 0.74 ± 0.04
5 ribosomes0.85 ± 0.03; 0.82 ± 0.020.83 ± 0.08; 0.73 ± 0.04
6 ribosomes0.84 ± 0.01; 0.81 ± 0.010.82 ± 0.08; 0.72 ± 0.07
  • Quantification of TDP-43 protein distribution throughout the polysome gradient relative to S6 ribosomal protein distribution.