Table 1.

Antibodies and dilutions used for histological analysis in this study

AntigenNameDilutionCatalogCompany
BrdURat anti-BrdU1:200MCA2060Serotec
CNPaseMouse anti-CNPase1:1000SMI-91RCovance
GFAPRabbit anti-GFAP1:800ab5804Millipore
hGFAPMouse antihuman (specific) GFAP1:500SMI-21RCovance
hNMouse antihuman nuclei, clone 235-11:800MAB1281Millipore
hNG2Mouse anti-NG2, clone 9.2.271:200MAB2029Millipore
Ki67Rabbit anti-Ki67, clone SP61:200RM-9106-S1LabVision
MBPRat anti-MBP1:25ab7349Abcam
mNG2Rabbit anti-NG21:200AB5320Millipore
Olig2Rabbit anti-Olig21:500RA25017Neuromics
PDGFRαRabbit anti- PDGFRα, clone D13C61:3005241SCell Signaling
PDGFRαRabbit anti- PDGFRα, clone D1E1E1:3003174SCell Signaling
TransferrinTransferrin antibody1:800ab9538Abcam
EGFPEGFP, 3E61:400A11120Invitrogen
Secondary antibodiesAlexaFluor-568 goat anti-mouse IgG (H + L)1:400A-11031Invitrogen
AlexaFluor-568 goat anti-mouse IgG11:400A-21124Invitrogen
AlexaFluor-488 goat anti-mouse IgG (H + L)1:400A-11029Invitrogen
AlexaFluor-488 goat anti-mouse IgG11:400A-21121Invitrogen
DyLight 649 goat anti-mouse IgG11:400115-495-205The Jackson Laboratory
Biotin-SP goat anti-mouse IgG (H + L)1:250115-065-166The Jackson Laboratory
AlexaFluor-568 goat anti-rabbit IgG (H + L)1:400A-11036Invitrogen
AlexaFluor-488 goat anti-rabbit IgG (H + L)1:400A-11034Invitrogen
Cy5 goat anti-rat1:400A10525Invitrogen
AlexaFluor-568 goat anti-rat IgG (H + L)1:400A-11077Invitrogen
AlexaFluor-488 goat anti-rat IgG (H + L)1:400A-11006Invitrogen
Streptavidin, AlexaFluor-5681:1000S-11226Invitrogen
Avidin, AlexaFluor-4881:500A-21370Invitrogen