Overview of IVIS imaging studies of mice that received intracarotid or intravenous injection with the diagnostic probes scFv235 or 6B2G12 antibody
| Objective | Conjugated dye | Injection route/probe | Mice | Results | |
|---|---|---|---|---|---|
| Uptake of scFv235 in the brain and binding to pathological tau within neurons | Alexa Fluor 568 | Intracarotid/scFv235 | 3 htau/PS1 | Good scFv signal that colocalizes well with Tau5 and MC1 antibody | |
| Intracarotid/scFv235 | 2 WT | Limited scFv signal | |||
| IVIS signals | Staining signal | ||||
| Live IVIS imaging for scFv235/6B2G12 | VivoTag 680XL | Intracarotid/scFv235 | 3 htau/PS1 | Imaging not performed for B32 mouse | Strong scFv signal that colocalizes well with tau aggregates and endosome/autophagosome/lysosome (e/a/L) markers for B32 mouse |
| Weak signal for BB5 mouse | Weak scFv signal and no tau pathology for BB5 mouse | ||||
| Strong signal in the right hemisphere that slowly decreases up to 4 h for A47 mouse | Strong scFv signal that colocalizes well with tau aggregates and e/a/L markers for A47 mouse | ||||
| Intracarotid/scFv235 | 4 P301L | Strong scFv signal that is sustained even after 5 h | Strong scFv signal that colocalizes well with tau aggregates and e/a/L markers | ||
| Intracarotid/scFv235 | 2 WT and 1 htau | Weak signal | Weak scFv signal. Limited (htau) or no (WT) tau pathology | ||
| Intracarotid/free dye | 2 P301L | Modest signal | Weak signal that does not colocalize with tau aggregates | ||
| Intracarotid/6B2G12 | 3 P301L | Strong signal that slowly decreases the next 3–5 h | Strong 6B2G12 signal that colocalizes well with tau aggregates and e/a/L markers | ||
| Intracarotid/6B2G12 | 3 WT | Weak signal | Weak 6B2G12 signal; no tau pathology | ||
| Intravenous/scFv235 | 3 P301L | Strong signal up to 1 h that slowly decreases the next 3 h | Weak scFv signal after 7–31 d that colocalizes well with tau aggregates and e/a/L markers | ||
| Intravenous/scFv235 | 1 TgSw DI, 1 WT | Weak signal | Weak scFv and tau antibody signals | ||
| Intravenous/6B2G12 | 3 P301L | Strong signal up to 1 h that slowly decreases the next 3 h | Weak signal after 7–31 d that colocalizes well with tau aggregates and e/a/L markers | ||
| Intravenous/6B2G12 | 1 TgSw DI, 1 WT | Weak signal | Weak 6B2G12 signal; no tau pathology | ||
| Intravenous/control IgG | 2 P301L | Modest signal | Weak control IgG signal; extensive tau pathology but IgG signal does not colocalize with tau aggregates | ||