Membrane properties of WT and SOD1G93A TMNs classified by predicted MU type
| Membrane property | PS | PFR | PFF | |||
|---|---|---|---|---|---|---|
| WT (n = 16) | SOD1G93A (n = 12) | WT (n = 16) | SOD1G93A (n = 9) | WT (n = 16) | SOD1G93A (n = 15) | |
| Cm, pF | 115.0 ± 8.4 | 114.0 ± 7.8 | 142.2 ± 11.0 | 111.7 ± 8.3 | 185.6 ± 21.2 | 144.7 ± 10.5 |
| Rinp, MΩ | 132.8 ± 12.8 | 131.7 ± 8.4 | 59.1 ± 3.9 | 78.6 ± 5.3* | 35.7 ± 2.2 | 51.4 ± 3.0** |
| RMP, mV | −61.6 ± 1.8 | −60.1 ± 1.6 | −65.5 ± 1.2 | −65.9 ± 2.4 | −67.4 ± 1.6 | −64.4 ± 1.5 |
| Rheobase, pA | 151.6 ± 13.8 | 123.8 ± 13.8 | 459.1 ± 30.2 | 274.4 ± 16.5† | 800.7 ± 67.3 | 539.3 ± 19.1†† |
| AP threshold, mV | −40.0 ± 1.3 | −39.4 ± 1.2 | −33.5 ± 1.3 | −36.5 ± 2.6 | −33.5 ± 2.3 | −31.2 ± 1.3 |
| AP height, mV | 83.5 ± 2.8 | 85.7 ± 2.8 | 79.1 ± 2.2 | 83.3 ± 3.7 | 82.1 ± 2.6 | 73.8 ± 1.5‡ |
| AP half-width, ms | 1.0 ± 0.05 | 0.9 ± 0.1 | 0.8 ± 0.04 | 1.0 ± 0.1# | 0.7 ± 0.07 | 0.8 ± 0.0 |
| AHP, mV | −5.4 ± 1.3 | −3.8 ± 1.1 | −4.0 ± 1.1 | −5.4 ± 1.5 | −3.4 ± 1.1 | −3.4 ± 0.7 |
Values reported as mean ± SEM. Student t tests were performed for statistical comparison between WT and SOD1G93A neurons within the three predicted MU types. Symbols indicate statistical significance, where:
↵*p = 0.007,
↵**p = 0.003,
↵†p = 0.00024,
↵††p = 7.83712E-05,
↵‡p = 0.0074,
↵#p = 0.032. Bold face highlights values that show statistical significance in the mutant.