Antibodies used in this study
| Antibody | Species | Company (reference) | Dilutions | ||
|---|---|---|---|---|---|
| IF | WB | IHC/EM | |||
| Anti-Chmp2b | Rabbit polyclonal | Abcam (ab33174) | 500 | 10,000 | 1000 |
| Anti-Chmp2b | Mouse monoclonal | Covalab | 100 | 2000 | |
| Anti-Chmp4b | Rabbit polyclonal | Abcam (ab105767) | 500 | 2000 | |
| Anti-Chmp4b | Mouse monoclonal | Covalab | 100 | 1000 | |
| Anti-Alix | Rabbit polyclonal | Covalab (ab0204) | 6000 | ||
| Anti-PSD95 | Mouse monoclonal | NeuroMab (73-028) | 200 | 4000 | |
| Anti-GluN1 | Mouse monoclonal | Synaptic Systems (114011) | 200 | 4000 | |
| Anti-Synaptophysin | Mouse monoclonal | Merck Millipore (MAB5258) | 10,000 | ||
| Anti-HA | Mouse monoclonal | Cell Signaling (2367) | 200 | 5000 | |
| Anti-HA | Rabbit polyclonal | Sigma-Aldrich (H6908) | 5000 | ||
| Anti-Flag | Mouse monoclonal | Sigma-Aldrich (F3165) | 1000 | 10,000 | |
| Anti-Flag | Rabbit polyclonal | Sigma-Aldrich (F7425) | 1000 | 5000 | |
| Anti-Actin | Mouse monoclonal | Millipore (MAB1501R) | 20,000 | ||
| Anti-Tubulin | Mouse monoclonal | Gift from A. Andrieux's laboratory | 20,000 | ||
| Anti-Pan MAGUK | Mouse monoclonal | NeuroMab (75-029) | 4000 | ||
| Anti-GluN2B | Mouse monoclonal | NeuroMab (75-097) | 4000 | ||
| Anti-Glu2 | Mouse monoclonal | Millipore (MABN71) | 5000 | ||
| Anti-ShankIII | Rabbit polyclonal | Santa Cruz Biotechnology (30193) | 1000 | ||
| Anti-Homer | Mouse monoclonal | Santa Cruz Biotechnology (17842) | 2000 | ||
| Anti-Mouse HRP | Goat polyclonal | Jackson ImmunoResearch (115-035-166) | 20,000 | ||
| Anti-Rabbit HRP | Goat polyclonal | Jackson ImmunoResearch (115-035-044) | 20,000 | ||
| Anti-Mouse Alexa Fluor 488 | Goat polyclonal | Invitrogen (A-11029) | 1500 | ||
| Anti-Mouse Alexa Fluor 594 | Goat polyclonal | Invitrogen (A-11032) | 1500 | ||
| Anti-Mouse Cy5 | Goat polyclonal | Jackson ImmunoResearch (115-175-146) | 1000 | ||
| Anti-Rabbit Alexa Fluor 488 | Goat polyclonal | Invitrogen (A-11034) | 1500 | ||
| Anti-Rabbit Alexa Fluor 594 | Goat polyclonal | Invitrogen (A-11037) | 1500 | ||
| Anti-Rabbit Cy5 | Goat polyclonal | Jackson ImmunoResearch (111-175-144) | 1000 | ||
The antibody dilutions we used are indicated for each application. IF, Immunofluorescence; WB, Western immunoblotting; IHC, immunohistochemistry.