Table 2.

Fos-positive/Fos-negative ratio gene expression in each context

GeneRatio: Fos-positive/Fos-negativeaMain effect of Fos cell typeMain effect of ContextInteraction
Extinction Context BMethamphetamine Context A
Neuronal marker
    NeuN1.1, n = 91.2, n = 13F(1, 20) = 8.4, p < 0.01F(1, 20) = 0.1, p = 0.91F(1, 20) = 1.3, p = 0.26
IEGs
    Fosb4.7, n = 72.5, n = 8F(1, 13) = 13, p < 0.01F(1, 13) = 0.4, p = 0.55F(1, 13) = 0.2, p = 0.67
    Total FosBFosBb8.1, n = 32.6, n = 9F(1, 10) = 29, p < 0.01F(1, 10) = 0.61, p = 0.45F(1, 10) = 4.1, p = 0.07
    ΔFosB1.0, n = 44.3, n = 10F(1,12) = 1.2, p = 0.29F(1, 12) = 0.1, p = 0.82F(1, 12) = 1.2, p = 0.29
    ΔFosB-21.7, n = 110.9, n = 13F(1, 22) = 0.9, p = 0.36F(1, 22) = 1.6, p = 0.21F(1, 22) = 1.2, p = 0.29
    Arc1.6, n = 101.2, n = 13F(1, 21) = 1.7, p = 0.20F(1, 21) = 0.1, p = 0.92F(1, 21) = 0.4, p = 0.53
    Homer1b1.6, n = 101.3, n = 13F(1, 21) = 5.6, p < 0.05F(1, 21) = 0.7, p = 0.40F(1, 21) = 0.8, p = 0.36
    Homer2b4.3, n = 72.0, n = 7F(1, 12) = 14, p < 0.01F(1, 12) = 3.7, p = 0.07F(1, 12) = 4.4, p = 0.06
Receptors
    Drd11.3, n = 71.0, n = 13F(1, 18) = 0.5, p = 0.48F(1, 18) = 1.2, p = 0.22F(1, 18) = 0.7, p = 0.41
    Drd21.0, n = 91.2, n = 13F(1, 20) = 1.6, p = 0.22F(1, 20) = 3.9, p = 0.06F(1, 20) = 1.9, p = 0.18
    Grin1 (GluN1)1.0, n = 111.0, n = 13F(1, 22) = 0.2, p = 0.63F(1, 22) = 0.6, p = 0.44F(1, 22) = 1.9, p = 0.19
    Grin2a (GluN2a)b1.4, n = 121.6, n = 17F(1, 27) = 13, p < 0.01F(1, 27) = 1.2, p = 0.28F(1, 27) = 0.5, p = 0.47
    Gria1 (GluA1)1.0, n = 111.1, n = 13F(1, 22) = 1.5, p = 0.23F(1, 22) = 0.1, p = 0.94F(1, 22) = 0.5, p = 0.51
    Gria2 (GluA2)1.2, n = 111.0, n = 13F(1, 22) = 6.1, p < 0.05F(1, 22) = 0.5, p = 0.49F(1, 22) = 0.1, p = 0.79
    Cb1rb1.7, n = 121.4, n = 14F(1, 24) = 16, p < 0.01F(1, 24) = 0.1, p = 0.96F(1, 24) = 1.2, p = 0.28
    Trkb11.6, n = 61.0, n = 6F(1, 10) = 3.0, p = 0.11F(1, 10) = 0.1, p = 0.95F(1, 10) = 1.0, p = 0.33
Other genes
    Darpp-321.3, n = 111.1, n = 13F(1, 22) = 1.7, p = 0.20F(1, 22) = 0.8, p = 0.37F(1, 22) = 0.3, p = 0.59
    Mapk2b0.8, n = 81.2, n = 13F(1, 19) = 0.1, p = 0.72F(1, 19) = 3.3, p = 0.08F(1, 19) = 3.9, p = 0.06
    Mecp2_A20.9, n = 111.0, n = 13F(1, 22) = 0.4, p = 0.55F(1, 22) = 0.1, p = 0.86F(1, 22) = 0.5, p = 0.49
  • aUsing 2e(−ΔΔCt) values from only matched pairs Fos-positive and Fos-negative within the same animal.

  • bGenes that also passed the criterion of >1.5 for expression in Fos-positive over Fos-negative neurons.