Summary of channel gating parameters for WT and mutant NaV1.7 channelsa
| WT | W1775R | A1236E | L1831X | L245V | β1/β2 only | |
|---|---|---|---|---|---|---|
| Activation | ||||||
| V0.5 (mV) | −25.2 ± 1.6 | −13.4 ± 1.3, p = 5.24E-4 | −21.5 ± 1.8 | −15.4 ± 1.8, p = 1.50E-3 | −25.2 ± 1.3 | −30.3 ± 4.9 |
| k | −3.7 ± 0.3 | −7.5 ± 0.2, p = 1.54E-6 | −7.3 ± 0.4, p = 3.54E-6 | −7.6 ± 0.2, p = 1.30E-7 | −4.9 ± 0.4, p = 2.16E-2 | −9.5 ± 1.2, p = 6.72E-7 |
| VRev (mV) | 67.5 ± 2.3 | 71.4 ± 4.9 | 67.5 ± 3.8 | 72.5 ± 5.3 | 74.0 ± 3.1 | 76.9 ± 15.5 |
| Imax (pA.pF−1) | −361.2 ± 39.5 | −115.9 ± 15.0, p = 2.24E-3 | −41.0 ± 11.9, p = 5.75E-5 | −103.3 ± 14.0, p = 3.73E-4 | −398.5 ± 44.7 | −8.9 ± 1.1, p = 1.18E-4 |
| n | 23 | 7 | 8 | 9 | 16 | 6 |
| Fast inactivation | ||||||
| V0.5 (mV) | −71.1 ± 1.3 | −73.4 ± 2.2 | −80.7 ± 1.3, p = 3.22E-4 | −82.2 ± 1.3, p = 2.82E-5 | — | −72.6 ± 4.4 |
| k | 6.1 ± 0.4 | 11.6 ± 1.1, p = 1.68E-5 | 9.1 ± 0.8, p = 1.91E-3 | 7.9 ± 0.6, p = 2.38E-2 | — | 8.2 ± 1.7 |
| n | 22 | 7 | 8 | 9 | 6 | |
| DB fast inactivation | ||||||
| V0.5 (mV) #1 | — | — | — | — | 71.2 ± 0.6 | — |
| V0.5 (mV) #2 | — | — | — | — | 31.4 ± 1.8 | — |
| n | 10 | |||||
| Slow inactivation | ||||||
| V0.5 (mV) | −31.4 ± 1.8 | — | — | — | −41.4 ± 0.9, p = 4.00E-4 | |
| k | 9.9 ± 0.6 | — | — | — | 11.3 ± 1.7 | — |
| n | 8 | 16 | ||||
↵aThe table shows averaged parameters for channel activation, fast inactivation, and slow inactivation for WT and mutant NaV1.7 channels (W1775R, A1236E, L1831X, and L245V), as well as the β1/β2 subunit. Fast inactivation values for L245V are under a separate entry because of the double Boltzmann fit (DB fast inactivation). Data are mean ± SEM. All values are compared with the averaged WT value. p value is given for comparisons that are deemed significantly different (p < 0.05). All single-parameter comparisons were tested using an unpaired two-way Student's t test.