Table 3.

Statistics for mRNA expression of genes in both homogenate dorsal striatum and FACS sorted dorsal striatal neurons

GenesWhole DS extracts (one-way ANOVA)FACS
Paired t test (extinction test; Fos positive vs Fos negative)Unpaired t test (Fos negative; no test vs extinction test)
Immediate early genes
    Fost(14) = 5.5, p = 0.00008*t(22) = 3.0, p = 0.006
    Egr1t(13) = 4.2, p = 0.001*t(21) = 0.6, p = 0.5
    Arct(14) = 5.3, p = 0.0001*t(22) = 0.2, p = 0.2
Epigenetic enzyme genes
    Hdac1F(2,20) = 0.1, p = 0.9t(13) = 1.3, p = 0.2t(22) = 2.2, p = 0.04
    Hdac2F(2,19) = 1.6, p = 0.2t(14) = 2.5, p = 0.03t(23) = 1.8, p = 0.1
    Hdac3F(2,20) = 1.0, p = 0.4t(13) = 3.7, p = 0.003*t(22) = 0.4, p = 0.7
    Hdac4F(2,18) = 1.1, p = 0.5t(13) = 5.6, p = 0.00008*t(21) = 1.7, p = 0.1
    Hdac5F(2,20) = 4.5 p = 0.02t(14) = 4.4, p = 0.0007*t(22) = 0.6, p = 0.5
    Sirt1F(2,20) = 1.1, p = 0.3t(13) = 1.4, p = 0.2t(22) = 1.1, p = 0.3
    Sirt2F(2,20) = 0.2, p = 0.8t(13) = 2.4, p = 0.03t(22) = 1.8, p = 0.1
    CrebbpF(2,20) = 1.9, p = 0.2t(13) = 2.8, p = 0.01t(22) = 1.4, p = 0.2
    Suv 39h1F(2,19) = 1.2, p = 0.3t(11) = 1.9, p = 0.09t(20) = 1.8, p = 0.1
    G9aF(2,20) = 0.8, p = 0.5t(14) = 0.1, p = 0.9t(22) = 1.4, p = 0.2
    GLPF(2,20) = 0.9, p = 0.4t(13) = 4.0, p = 0.002*t(22) = 2.5, p = 0.02
    Kdm1aF(2,20) = 2.7, p = 0.1t(13) = 3.9, p = 0.002*t(22) = 2.4, p = 0.02
    Mll1F(2,20) = 7.1, p = 0.0048*t(14) = 1.6, p = 0.1t(22) = 0.4, p = 0.7
    Dnmt3aF(2,19) = 0.9 p = 0.4t(13) = 8.3, p = 0.0001*t(22) = 2.6, p = 0.01
Glutamate receptor genes
    Gria1F(2,20) = 1.1, p = 0.4t(13) = 3.9, p = 0.002*t(22) = 1.3, p = 0.2
    Gria2F(2,20) = 0.4, p = 0.6t(14) = 3.9, p = 0.002*t(23) = 1.6, p = 0.1
    Gria3F(2,18) = 0.1, p = 0.9t(13) = 3.4, p = 0.005*t(22) = 1.5, p = 0.1
    Grin1F(2,18) = 1.4, p = 0.3t(14) = 0.1, p = 1.0t(22) = 0.6, p = 0.6
    Grin2aF(2,17) = 0.9, p = 0.4t(14) = 4.3, p = 0.0007*t(23) = 0.4, p = 0.7
    Grin2bF(2,19) = 1.4, p = 0.3t(14) = 2.5, p = 0.03t(22) = 0.1, p = 0.9
    Grm1F(2,17) = 4.9, p = 0.02t(12) = 5.4, p = 0.0002*t(21) = 0.4, p = 0.7
    Grm5F(2,20) = 0.8, p = 0.4t(14) = 0.2, p = 0.9t(23) = 0.6, p = 0.5
Growth factor genes
    BdnfF(2,18) = 0.1, p = 0.9t(13) = 6.1, p = 0.00004*t(22) = 2.1, p = 0.04
    TrkbF(2,19) = 3.1, p = 0.07t(11) = 3.6, p = 0.004*t(19) = 0.2, p = 0.8
  • We analyzed the data of the whole DS extracts using a between-subjects factor one-way ANOVA including three groups: saline (combined data of saline day 2 and day 35), methamphetamine day 2, and methamphetamine day 35. We analyzed the FACS data using a within-subjects paired t test comparing Fos-positive to Fos-negative neurons in the extinction test group (n = 15). We also analyzed the FACS data using a between-subjects unpaired t test comparing Fos-negative neurons between the no test (n = 10) and extinction test groups.

  • *Significant group differences for whole DS extracts, and significant difference between Fos-positive and Fos-negative cells for FACS sorting, p < 0.005.