Table 6.

RAD-23 has opposing roles on substrate stabilizationa

SubstrateRAD-23 manipulationEffect on substratePlatformReference
Arg-β-GalOverexpressionStabilizesYeastOrtolan et al., 2000
Rad-4OverexpressionStabilizesYeastOrtolan et al., 2004
Ub-Pro-β-GalOverexpressionStabilizesYeastOrtolan et al., 2000
LactalbuminReplacementStabilizesUb-depleted reticulocyte extractRaasi and Pickart, 2003
Ub5-DHFRReplacementStabilizesHEK293T cellsRaasi and Pickart, 2003
BCA2OverexpressionStabilizesU202 and H1299 cellsBacopulus et al., 2012
p53OverexpressionStabilizesU202 cellsBrignone et al., 2004
p53Loss of function (hR23A and hR23B)DestabilizesU202 cellsBrignone et al., 2004
Ub-Pro-β-GalLoss of functionStabilizesΔrad23 yeastLambertson et al., 1999
UbV76-V-β-galLoss of functionStabilizesΔrad23 yeastRao et al., 2002; Kim et al., 2004
Sic1ReplacementDestabilizesProteasomes purified from Δrad23 yeastVerma et al., 2004
Far1Loss of functionStabilizesΔrad23 yeastVerma et al., 2004

  • ↵aSummary of past findings on RAD-23 manipulation and substrate turnover.