Table 1.

Candidate mutants for suppression of FLP-13 and NLP-22 overexpression studied in Figures 3 and 4, their vertebrate homologs, and the reasons that these mutants were selected for study

Gene mutated	Change in function	Vertebrate homolog	Reason chosen	Reference
gsa-1	Gain	Gαs	Reduced lethargus quiescence; resistant to isoflurane	(Saifee et al., 2011; Schwarz and Bringmann, 2013)
acy-1	Gain	Adenylate cyclase	Reduced lethargus quiescence	(Iwanir et al., 2013)
pde-4	Loss	PDE4	Reduced lethargus quiescence	(Singh et al., 2014)
kin-2	Loss	PKA regulatory subunit	Reduced lethargus quiescence; resistant to isoflurane	(Saifee et al., 2011; Belfer et al., 2013)
goa-1	DN	Gαi/o	Reduced lethargus quiescence	(Singh et al., 2014)
goa-1	Loss	Gαi/o	Resistant to halothane	(van Swinderen et al., 2001)
egl-30	Gain	Gαq	Reduced lethargus quiescence; resistant to halothane	(Hawasli et al., 2004; Schwarz and Bringmann, 2013)
eat-16	Loss	Gαq RGS	Resistant to halothane and isoflurane	(van Swinderen et al., 2001)
dgk-1	Loss	DGK-θ	Resistant to halothane	(van Swinderen et al., 2002)
gpa-12	Loss	Gα12	Activation inhibits feeding	(van der Linden et al., 2003)
tpa-1	Loss	PKCδ	Suppresses effects of gpa-12 activation	(van der Linden et al., 2003)
eat-6	Loss	Na⁺/K⁺ ATPase	Depolarized pharyngeal muscle	(Davis et al., 1995)
egl-19	Gain	L-type Ca²⁺ channel	Prolonged pharyngeal action potential	(Lee et al., 1997)
tom-1	Loss	Tomosyn	Increased neurotransmission	(Dybbs et al., 2005)
slo-1	Loss	BK channel	Increased neurotransmission; resistant to halothane	(Wang et al., 2001; Hawasli et al., 2004)
unc-77	Gain	NALCN channel	Resistant to halothane	(Humphrey et al., 2007)
unc-64	Neomorph	Syntaxin	Resistant to halothane and isoflurane	(van Swinderen et al., 1999)
unc-7	Loss	Innexin	Resistant to halothane	(Morgan et al., 1990)
tbh-1	Loss	Tyramine β-hydroxylase	Opposes 5-HT response	(Wragg et al., 2007)
egl-4	Loss	PKG	Reduced lethargus quiescence	(Raizen et al., 2008)

DN, Dominant-negative.