Table 5.

Two-way ANOVA of the data shown in Figure 6 (pENK, pOMC, and PDYN KO mice) and Figure 7 (MOR internalization)

RowFigureExperimentnVariable 1Variable 2InteractionSubject matching
16ApENK KO, CFAKO: 10Time (CFA)Genotypep = 0.83p = 0.0093
WT: 5p < 0.0001p = 0.048F(6,78) = 0.47F(13,78) = 2.39
F(6,78) = 9.52F(1,13) = 4.78
2pENK KO, baselineKO: 10TimeGenotypep = 0.18p = 0.0006
WT: 5p = 0.49p = 0.48F(3,39) = 1.70F(13,39) = 3.77
F(3,39) = 0.81F(1,13) = 0.53
36BpENK KO, NTXKO: 10Time (NTX)Genotypep = 0.88p = 0.014
WT: 5p < 0.0001p = 0.229F(3,39) = 0.23F(13,39) = 2.48
F(3,39) = 0.23F(1,13) = 1.59
46CpOMC KO, CFAKO: 4Time (CFA)Genotypep = 0.68p = 0.016
WT: 5p < 0.0001p = 0.99F(7,49) = 0.69F(7,49) = 2.79
F(7,49) = 7.7F(1,7) = 0.00001
5pOMC KO, baselineKO: 4TimeGenotypep = 0.06p < 0.0001
WT: 5p = 0.58p = 0.91F(9,36) = 2.0F(12,36) = 4.91
F(3,36) = 0.66F(3,12) = 0.18
66DpOMC KO, NTXKO: 4Time (NTX)Genotypep = 0.67p = 0.04
WT: 5p < 0.0001p = 0.23F(4,28) = 0.59F(7,28) = 2.48
F(4,28) = 8.8F(1,7) = 1.69
76EpDYN KO, CFAKO: 8Time (CFA)Genotypep = 0.51p < 0.0001
WT: 8p < 0.0001p = 0.037F(8,112) = 0.91F(14,112) = 5.38
F(8,112) = 11.38F(1,14) = 5.34
8pDYN, baselineKO: 8TimeGenotypep = 0.51p = 0.79
WT: 8p = 0.16p = 0.0069F(2,28) = 0.68F(14,28) = 0.66
F(2,28) = 1.94F(1,14) = 10.0
96FpDYN KO, NTXKO: 8Time (NTX)Genotypep = 0.25p < 0.0001
WT: 8p < 0.0001p = 0.45F(5,70) = 1.37F(14,70) = 6.40
F(5,70) = 9.47F(1,14) = 0.61
106GpDYN KO, JDTicKO: 8Time (JDTic)Genotypep = 0.91p < 0.0001
WT: 8p < 0.0001p = 0.55F(2,26) = 0.096F(13,26) = 6.90
F(2,26) = 14.60F(1,13) = 0.38
117ARats CFA6Time (CFA)Sidep < 0.0001p < 0.0001
p < 0.0001p = 0.0007F(7,70) = 17.4F(10,70) = 5.98
F(7,70) = 20.2F(1,10) = 23.4
127BRats NTX6Time (NTX)Sidep = 0.39p < 0.0001
p < 0.0001p = 0.92F(8,80) = 14.9F(10,80) = 4.7
F(8,80) = 14.9F(1,10) = 0.01
  • Each panel in Figure 6 or 7 (indicated in the second column) was analyzed independently by two-way ANOVA except for rows 2, 5, and 8 which are baseline data obtained before the CFA injection. In the fourth column, n is the number of animals in the experiment (KO or their WT littermates). In the fifth and sixth columns, the variable is identified first: “time” refers to the time after the injection of the compound in parentheses; “genotype” is KO versus WT, and “side” is ipsilateral versus contralateral paw. Subscript numbers in the F ratio are the degrees of freedom and the residual.