Table 1.

Summary of SPR experiments to screen for ApoE-binding sites within the SirT1 promoter sequence

DNA sequence name	DNA sequence	Variant	K_D ApoE3	K_D ApoE4	K_D trx
ApoE binding sequence	`cagcctccgcccgccacgtgacccgtagtg`	Forward	NS	NS	NS
		Complement	NS	NS	NS
		Double- stranded	3 nm	3 nm	NS
ApoE binding sequence-scrambled	`ccctgcgctgtgccgccaaacctgacgccg`	Forward	NS	NS	NS
		Complement	NS	NS	NS
		Double- stranded	NS	NS	NS
Fragment 2	`tcctgcccctagcggcct`	Forward	NS	NS	NS
		Complement	NS	NS	NS
		Double- stranded	NS	NS	NS
Fragment 3	`ggaagctga`	Forward	NS	NS	NS
		Complement	NS	NS	NS
		Double- stranded	NS	NS	NS
Fragment 4	`ggcggacg`	Forward	NS	NS	NS
		Complement	NS	NS	NS
		Double- stranded	NS	NS	NS
Fragment 5	`Ccacgtc`	Forward	NS	NS	NS
		Complement	NS	NS	NS
		Double- stranded	NS	NS	NS
Fragment 6	`ccgccacgtga`	Forward	NS	NS	NS
		Complement	NS	NS	NS
		Double- stranded	NS	NS	NS
Fragment 7	`ggtcacgtg`	Forward	NS	NS	NS
		Complement	NS	NS	NS
		Double- stranded	NS	NS	NS
Potential RelA binding sequence	`gtggaaattcc`	Forward	NS	NS	NS
		Complement	NS	NS	NS
		Double- stranded	NS	NS	NS
Potential RelA binding sequence-scrambled	`gtagagtacact`	Forward	NS	NS	NS
		Complement	NS	NS	NS
		Double- stranded	NS	NS	NS