Table 1.

Secondary motoneuron defects in col15a1b morphantsa

52 hpf72 hpf
TotalRoP-like nerveTotalRoP-like nerveMiP-like nerveCaP-like nerveCommon nerve
embryosAbsenceembryosAbsenceVentral projectionBranchingAbsenceAbsenceBranching
Uninjected4919.59 ± 4.044415.45 ± 2.920.45 ± 0.320.23 ± 0.230.68 ± 0.3800.23 ± 0.23
Ctl-MO5012.85 ± 2.31 (NS)476.80 ± 1.58 (NS)0.43 ± 0.29 (NS)0.43 ± 0.30 (NS)1.48 ± 0.6 (NS)0.21 ± 0.21 (NS)3.40 ± 1.15**
tMO15b5185.88 ± 3.79***5227.5 ± 3.73*9.03 ± 2.0***11.35 ± 2.18***59.04 ± 6.08***5.19 ± 2.10*6.35 ± 1.40***
sMO15b4768.94 ± 5.07***4429.32 ± 4.12**2.05 ± 0.62*2.95 ± 0.90***39.09 ± 5.90***3.18 ± 1.48*5.23 ± 1.36***
  • aQuantitative analysis of secondary motor axon/nerve defects per embryo under the different conditions. Results are expressed as mean frequencies of affected hemisegments per embryo ± SEM; 10 hemisegments per embryo were examined. NS, Nonsignificant.

  • *p < 0.05;

  • **p < 0.01;

  • ***p < 0.001; Student's t test.