52 hpf | 72 hpf | ||||||||
---|---|---|---|---|---|---|---|---|---|
Total | RoP-like nerve | Total | RoP-like nerve | MiP-like nerve | CaP-like nerve | Common nerve | |||
embryos | Absence | embryos | Absence | Ventral projection | Branching | Absence | Absence | Branching | |
Uninjected | 49 | 19.59 ± 4.04 | 44 | 15.45 ± 2.92 | 0.45 ± 0.32 | 0.23 ± 0.23 | 0.68 ± 0.38 | 0 | 0.23 ± 0.23 |
Ctl-MO | 50 | 12.85 ± 2.31 (NS) | 47 | 6.80 ± 1.58 (NS) | 0.43 ± 0.29 (NS) | 0.43 ± 0.30 (NS) | 1.48 ± 0.6 (NS) | 0.21 ± 0.21 (NS) | 3.40 ± 1.15** |
tMO15b | 51 | 85.88 ± 3.79*** | 52 | 27.5 ± 3.73* | 9.03 ± 2.0*** | 11.35 ± 2.18*** | 59.04 ± 6.08*** | 5.19 ± 2.10* | 6.35 ± 1.40*** |
sMO15b | 47 | 68.94 ± 5.07*** | 44 | 29.32 ± 4.12** | 2.05 ± 0.62* | 2.95 ± 0.90*** | 39.09 ± 5.90*** | 3.18 ± 1.48* | 5.23 ± 1.36*** |
↵aQuantitative analysis of secondary motor axon/nerve defects per embryo under the different conditions. Results are expressed as mean frequencies of affected hemisegments per embryo ± SEM; 10 hemisegments per embryo were examined. NS, Nonsignificant.
↵*p < 0.05;
↵**p < 0.01;
↵***p < 0.001; Student's t test.