Immunostaining of ASIC3 and P2X3 in functionally characterized muscle afferents recovered from the ex vivo recording preparations
| Response type | Condition | ASIC3+ | P2X3+ | ASIC3+/P2X3+ |
|---|---|---|---|---|
| Mechanoreceptors | Naive/sham | 3/5 | 3/6 | 3/6 |
| Control I/R | 4/5 | 2/5 | 1/5 | |
| PenIL1r1 + I/R | 0/6 | 2/6 | 0/6 | |
| PenASIC3 + I/R | 0/1 | 1/1 | 0/1 | |
| Metabo-nociceptors (high responders only) | Naive/sham | 3/4 | 3/4 | 2/4 |
| Control I/R | 1/5 | 3/5 | 1/5 | |
| PenIL1r1 + I/R | 2/3 | 0/3 | 0/3 | |
| PenASIC3 + I/R | 0/2 | 1/2 | 0/2 | |
| Metaboreceptors (low responders and both responders) | Naive/sham | 0/3 | 0/4 | 0/4 |
| Control I/R | 3/7 | 2/6 | 2/6 | |
| PenIL1r1 + I/R | 0/4 | 2/4 | 0/4 | |
| PenASIC3 + I/R | 0/3 | 0/3 | 0/3 |
Italics indicate discrepancies in ASIC3 expression between conditions.