CNO-induced changes in active electrophysiological properties in neurons from V1Gi-DREAD mice (n = 15) and littermate controls (n = 10)
| Control | V1Gi-DREADD | |||
|---|---|---|---|---|
| Before CNO | After CNO | Before CNO | After CNO | |
| AP overshoot (mV)# | 51.4 ± 1.3 | 51.3 ± 0.6 | 42.3 ± 6.0 | 41.2 ± 4.3 |
| Duration (ms)¥ | 4.7 ± 0.3 | 4.8 ± 0.3 | 6.4 ± 1.2 | 4.6 ± 0.6** |
| AHP (mV)¥¥ | −12.9 ± 2.4 | −13.9 ± 1.7 | −18.3 ± 1.4 | −11.2 ± 1.4** |
| AHP decay (t, ms) | 241.1 ± 73.4 | 174.2 ± 55.1 | 106.1 ± 11.4 | 126.6 ± 23.1 |
A single 4 ms depolarizing current injection was used to measure CNO-induced changes in active properties as defined in Fig. 4A. Two-way ANOVA with Holm–Sidak post hoc analysis assessed active properties.
↵#p < 0.05, main effect of genotype;
↵¥ p < 0.05, interaction between genotype and CNO treatment;
↵¥¥ p < 0.01, interaction between genotype and CNO treatment.
↵**p < 0.01, post hoc comparison of CNO treatment within genotype.