Table 1.

Papaverine-inhibited PDE activity in different regions of the basal ganglia in NT, hWT, and hMT mice

Brain regionNThWThMT
Caudate–putamen0.878 ± 0.1360.743 ± 0.1790.755 ± 0.141
Globus pallidus0.034 ± 0.0140.046 ± 0.016*0.088 ± 0.035**
Entopeduncular nucleus0.054 ± 0.0260.028 ± 0.009*0.020 ± 0.013*
Substantia nigra0.37 ± 0.280.26 ± 0.110.22 ± 0.17
  • Total PDE activity was assayed in the presence of 0.1 μm cAMP; papaverine-inhibited PDE activity was evaluated after adding a fixed dose of papaverine (0.2 μm). PDE activity was detected as nmol cAMP hydrolyzed/min/mg protein. Papaverine-inhibited PDE activity was calculated as the difference between the basal PDE activity and the residual PDE activity assayed in the presence of papaverine. N = 6 in each group. One-way ANOVA followed by Bonferroni's correction for multiple data. Caudate–putamen: no significant differences between NT, hWT, and hMT mice; globus pallidus: **p < 0.01 NT versus hMT, *p < 0.05 hWT versus hMT; entopeduncular nucleus: *p < 0.05 NT versus hWT, and versus hMT; substantia nigra: no significant differences between NT, hWT, and hMT mice.