Table 1.

C. elegans strains and transgenic lines used in this study

StrainGenotypeDescription
Bristol N2C. elegans wild-typeWild-type
Genetic ablation of neurons
wIs#3 Is [odr-1(gcy-10)::mCasp, odr-1(gcy-10)::GFP, mec-4::GFP]Genetic ablation of AWC
    OH8585otIs4[gcy-7p::gfp]; otEx3822[ceh-36::CZ-caspase3(p17), gcy-7::caspase3(p12)-NZ, myo-3::mCherry]Genetic ablation of ASEL
    JN580peIs580 [ins-1(short)p::casp1; ins-1(short)p::venus unc-122p::gfp]Genetic ablation of AIA
    JN579peIs579 [ttx-3p::casp1 ttx-3p::venus lin-44p::gfp]Genetic ablation of AIY
    JN578peIs578 [npr-9p::casp1 npr-9p::venus unc-122p::mCherry]Genetic ablation of AIB
    JN1693peIs579 [ttx-3p::mcasp1 ttx-3p::venus lin-44p::gfp]; peIs580 [ins-1(short)p::mcasp1 ins-1(short)::venus unc-122p::gfp]Genetic ablation of AIY and AIA
    JN604peIs578 [npr-9p::casp1 npr-9p::venus unc-122p::mCherry]; peIs579[ttx-3p::casp1 ttx-3p::venus lin-44p::gfp]Genetic ablation of AIY and AIB
    JN605peIs578 [npr-9p::casp1 npr-9p::venus unc-122p::mCherry]; peIs580[ins-1(short)p::casp1 ins-1(short)::gfp unc-122p::gfp]Genetic ablation of AIB and AIA
    JN1694peIs578 [npr-9p::mcasp1 npr-9p::venus unc-122p::mcherry]; peIs579 [ttx-3p::mcasp1 ttx-3p::venus lin-44p::gfp]; peIs580 [ins-1(short)p::mcasp1 ins-1(short)p::venus unc-122p::gfp]Genetic ablation of AIY, AIA, and AIB
Optogenetics
    JN1695lite-1(ce314) X; peIs1095[gcy-7p::ChR2::venus unc-122p::mCherry]Optical stimulation of ASEL
    JN1621lite-1(ce314) X; peIs1095[gcy-7p::ChR2::venus unc-122p::mCherry]; peIs578[npr-9p::casp1 npr-9p::venus unc-122p::mCherry]Optical stimulation of ASEL under genetic ablation of AIB
    JN1622lite-1(ce314) X; peIs1095[gcy-7p::ChR2::venus unc-122p::mCherry]; peIs579[ttx-3p::casp1 ttx-3p::venus lin-44p::gfp]Optical stimulation of ASEL under genetic ablation of AIY
    JN1623lite-1(ce314) X; peIs1095[gcy-7p::ChR2::venus unc-122p::mCherry]; peIs580[ins-1 (short)p::casp1 ins-1(short)p::venus unc-122p::gfp]Optical stimulation of ASEL under genetic ablation of AIA
    JN1696lite-1(ce314) X; Ex[npr-9p::ChR2 unc-122p::mcherry]Optical stimulation of AIB
    JN1605lite-1(ce314) X; Ex[ins-1 (short)p::ChR2 unc-122p::mCherry]Optical stimulation of AIA
    JN1697lite-1(ce314) X; Ex[ttx-3p::ChR2 unc-122p::mcherry] (#1)Optical stimulation of AIY (“#1 strain”)
    SRS281lite-1(ce314) X; pha-1 (e2123) III; sraEx281[ttx-3p::chop2(H134R)::TagRFP + pBX(pha-1(+))]Optical stimulation of AIY (“#2 strain”)
    JN1699lite-1(ce314) X; peIs1095 [gcy-7p::ChR2::venus unc-122p::mCherry]; dyf-11(pe554) X; Ex[gcy-7p::venus::dyf-11(+); myo-3p::Venus]Optical stimulation of ASEL; ASEL-specific rescue of dyf-11 (pe554)
    JN1698lite-1(ce314) dyf-11(pe554) X; peIs1095[gcy-7p::ChR2::venus unc-122p::mCherry]Optical stimulation of ASEL; dyf-11(pe554) background
    JN1701lite-1(ce314) X; peIs1095[gcy-7p::ChR2::venus unc-122p::mCherry]; Ex[gcy-7p::egl-30(Y61N) myo-3::venus]Optical stimulation of ASEL; ASEL-specific gain of function of egl-30
    JN1624lite-1(ce314) X; peIs1095[gcy-7p::ChR2::venus unc-122p::mCherry]; eat-4(ky5)IIIOptical stimulation of ASEL; eat-4(ky5)III background
    JN1629lite-1(ce314) X; peIs1095[gcy-7p::ChR2::venus unc-122p::mCherry]; Ex[gcy-7p::eat-4(RNAi); myo-3p::venus]Optical stimulation of ASEL; ASEL-specific RNAi of eat-4
Imaging and optogenetics
    JN609lite-1(ce314) X; peIs1095[gcy-7p::ChR2::venus unc-122p::mCherry]; Ex[npr-9p::RCaMP2; lin-44::gfp]Imaging of AIB under photoactivation of ASEL
    JN610lite-1(ce314) X; peIs1095[gcy-7p::ChR2::venus unc-122p::mCherry]; Ex[ttx-3p::RCaMP2; lin-44::gfp]Imaging of AIY under photoactivation of ASEL
    JN608lite-1(ce314) X; peIs1095[gcy-7p::ChR2::venus unc-122p::mCherry]; Ex[ins-1p::RGECO; lin-44::gfp]Imaging of AIA under photoactivation of ASEL
    JN611lite-1(ce314) X; peIs1095[gcy-7p::ChR2::venus unc-122p::mCherry]; Ex[gcy-7p::RCaMP2; lin-44::gfp]Imaging of ASEL under photoactivation of ASEL