Table 2.

Long-term drinking induces expression changes in proteins in the macaque OFC

Protein	Gene	Accession #	Peptides/spectra	F value	p value	Fold change	Coverage %	Mascot score
Cytoplasm
cAMP-dependent PKA C-α, Isoform 2	PRKACA	P17612-2	2/2	17.52	0.006	1.09	26.5	250
D-3-phosphoglycerate dehydrogenase	PHGDH	O43175	4/5	7.72	0.032	1.26	14.1	457
Dynamin-1^*	DNM1	Q05193	6/7	25.79	0.002	0.95	12.0	103
FAS-associated factor 2	FAF2	Q96CS3	4/5	6.94	0.039	1.19	24.3	263
Glial fibrillary acidic protein	GFAP	P14136	10/17	7.01	0.038	0.62	49.1	866
G(I) protein subunit α-1, Isoform 2^*	GNAI1	P63096-2	6/19	20.35	0.004	1.20	51.4	2977
G(I) protein subunit α-2, Isoform 3^*	GNAI2	P04899-3	6/15	8.91	0.025	1.13	55.5	2755
G(I)/G(S)/G(O) protein subunit γ-3^*	GNG3	P63215	4/17	8.11	0.029	1.16	72.0	908
G(I)/G(S)/G(T) protein subunit β-2^*	GNB2	P62879	2/2	11.8	0.014	1.24	44.7	11,797
G(k) protein subunit α^*	GNAI3	P08754	2/2	13.38	0.011	1.20	34.5	1566
Hemoglobin subunit β	HBB	P68871	2/3	7.24	0.036	0.31	34.7	353
MICOS complex subunit MIC19	CHCHD3	Q9NX63	10/14	6.65	0.042	0.78	53.3	543
Myosin light polypeptide 6	MYL6	P60660	4/6	8.63	0.026	0.55	13.9	161
Protein NipSnap homolog 3A	NIPSNAP3A	Q9UFN0	2/2	15.47	0.008	1.24	15.0	105
Endoplasmic reticulum membrane
Inositol 1,4,5-trisphosphate receptor type 1, Isoform 5^*	ITPR1	Q14643-5	3/3	7.21	0.036	1.16	4.2	417
Oxysterol-binding protein-related protein 8, Isoform 2	OSBPL8	Q9BZF1-2	3/3	6.92	0.039	0.85	6.1	176
Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 3	HACD3	Q9P035	2/3	7.81	0.031	0.79	14.9	343
α-Soluble NSF attachment protein	NAPA	P54920	3/3	6.82	0.040	1.19	44.4	271
Mitochondria
Amine oxidase (flavin-containing) B, Isoform 2	MAOB	P27338-2	8/43	6.58	0.043	1.30	36.0	3199
Calcium uptake protein 3, mitochondrial	MICU3	Q86XE3	2/3	41.1	0.001	0.82	7.4	233
cGMP-dependent phosphodiesterase, isoform PDE2A1	PDE2A	O00408-2	9/18	37.48	0.001	1.07	26.9	1345
Cytochrome b–c1 complex subunit Rieske	UQCRFS1	P47985	2/10	6.6	0.042	3.08	21.9	1305
Cytochrome c oxidase subunit 1	MT-CO1	P00395	2/2	7.51	0.034	1.20	4.9	127
Dihydrolipoyl dehydrogenase, mitochondrial, Isoform 3	DLD	P09622-3	6/19	7.91	0.031	1.16	25.9	1481
Glutamate dehydrogenase 1, mitochondrial^*	GLUD1	P00367	6/9	7.33	0.035	1.15	35.8	601
NAD(P) transhydrogenase, mitochondrial	NNT	Q13423	21/53	6.76	0.041	1.31	32.7	3472
Prohibitin-2	PHB2	Q99623	12/95	6.52	0.043	1.35	51.2	6861
Sideroflexin-1	SFXN1	Q9H9B4	8/14	7.01	0.038	1.30	51.9	1155
Solute carrier family 25 member 46	SLC25A46	Q96AG3	3/5	6.71	0.041	1.17	11.7	287
Tricarboxylate transport protein, mitochondrial	SLC25A1	P53007	3/5	6.96	0.039	0.82	29.3	225
Nucleus
Histone H4	HIST1H4A	P62805	2/2	9.89	0.020	0.40	40.8	68
Plasma membrane
4F2 cell-surface antigen heavy chain, Isoform 3	SLC3A2	P08195-3	3/6	6.23	0.047	1.23	8.2	231
Dipeptidyl aminopeptidase-like protein 6, Isoform DPPX-S	DPP6	P42658-2	2/4	9.86	0.020	1.14	7.4	163
FXYD domain-containing ion transport regulator 6	FXYD6	Q9H0Q3	2/7	6.99	0.038	1.28	50.5	280
Glutamate receptor 1, Isoform 4^*	GRIA1	P42261-4	4/4	9.23	0.023	1.23	13.2	442
Neuronal membrane glycoprotein M6-a^*	GPM6A	P51674	2/3	12.11	0.013	1.38	12.9	120
Neurotrimin, Isoform 4	NTM	Q9P121-4	6/19	7.25	0.036	1.13	34.9	776
Proline-rich transmembrane protein 2^*	PRRT2	Q7Z6L0	2/3	6.71	0.041	1.49	5.8	194
Ras-related protein Rab-5C	RAB5C	P51148	2/2	8.76	0.025	1.17	30.9	94
Ras-related protein R-Ras2^*	RRAS2	P62070	2/2	16.79	0.006	1.33	13.3	155
Reticulon-4 receptor-like 2	RTN4RL2	Q86UN3	3/4	7.17	0.037	1.18	13.1	258
Rho-related GTP-binding protein RhoB^*	RHOB	P62745	2/3	10.54	0.018	1.33	47.4	368
Na⁺/Ca²⁺ exchanger 2	SLC8A2	Q9UPR5	6/8	10.69	0.017	1.37	18.3	491
Na⁺/K⁺-transporting ATPase subunit α-1, isoform 2	ATP1A1	P05023-2	12/26	6.14	0.048	1.32	34.9	4315
Na⁺/K⁺-transporting ATPase subunit α-3, isoform 3	ATP1A3	P13637-3	13/84	6.55	0.043	1.27	41.4	7233
Na⁺/K⁺-transporting ATPase subunit β-2	ATP1B2	P14415	3/3	6.07	0.049	1.35	19.0	222
Solute carrier family 12 member 5, Isoform 2	SLC12A5	Q9H2X9-2	10/15	9.56	0.021	1.20	16.6	965
Synaptic vesicle membrane
Sodium-dependent neutral amino acid transporter^*	SLC6A17	Q9H1V8	2/2	13.42	0.011	1.30	8.0	288
Synaptic vesicle glycoprotein 2A, Isoform 2^*	SV2A	Q7L0J3-2	3/3	6.74	0.041	1.21	11.6	346
Synaptic vesicle glycoprotein 2B^*	SV2B	Q7L1I2	4/4	6.35	0.045	1.23	11.4	305
Synaptogyrin-1, isoform 1B^*	SYNGR1	O43759-2	2/2	8.44	0.027	1.35	10.3	95
Synaptogyrin-3^*	SYNGR3	O43761	2/5	8.92	0.024	1.27	11.8	337
Synaptosomal-associated protein 25, Isoform 2^*	SNAP25	P60880-2	2/2	16.45	0.007	1.28	64.1	1772
Syntaxin-12^*	STX12	Q86Y82	2/3	8.68	0.026	1.21	16.7	521
Syntaxin-1A, isoform 2^*	STX1A	Q16623-2	8/18	9.24	0.023	1.26	47.2	1468
Syntaxin-1B^*	STX1B	P61266	10/15	6.53	0.043	1.17	41.7	901
Vesicular glutamate transporter 1, Isoform 3^*	SLC17A7	Q9P2U7-3	2/5	6.27	0.046	1.21	12.7	249

↵*Denotes synaptic proteins depicted in Figure 3G.