PCR primer sequences and conditions
| Name | Species | Use | Forward (5′–3′) | Reverse (5′–3′) | Annealing temperature (°C) | Product size (bp) |
|---|---|---|---|---|---|---|
| mC5aR1 | Mouse | RT-PCR | ATGCTGATGCTGATGCTGATCG | ATGCTGATGGCTGATCGTCGGATGCTGAT | 60 | 562 |
| mActB | Mouse | RT-PCR | GTGGGCCGCCCTAGGCACCAG | CTCTTTGATGTCACGCACGATTTC | 60 | 103 |
| mC5aR1 | Mouse | qPCR | GGGATGTTGCAGCCCTTATCA | CGCCAGATTCAGAAACCAGATG | 60 | 131 |
| mSox2 | Mouse | qPCR | TAGAGCTAGACTCCGGGCGATGA | TTTCGTGGTCTTGTTTAAGGCAA | 60 | 296 |
| m18s | Mouse | qPCR | GATCCATTGGAGGGCAAGTCT | CCAAGATCCAACTACGAGCTT | 60 | 103 |
| hC5AR | Human | qPCR | TCCTTCAATTATACCACCCCTGA | GGAAGACGACTGCAAAGATGA | 60 | 139 |
| hC5 | Human | qPCR | ACTGAATTTGGTTGCTACTCCTC | GTATTACTGGGACTCCTCCTACC | 60 | 110 |
| hACTB | Human | qPCR | GCGGGAAATCGTGCGTGACATT | GATGGAGTTGAAGGTAGTTTGGTG | 60 | 232 |
| hCDH2 | Human | qPCR | ATCAACCCCATACACCAGCC | GTCGATTGGTTTGACCACGG | 60 | 128 |