Table 1.

Primers used for quantitative real-time PCR to assess mRNA expression of the five subtypes of dopamine receptors and β-actin

GeneAnnealing temperatureAccession numberProduct length (bp)Primer sequences
DRD155°CNM_012546.3168Forward: 5′-TCCTTCAAGAGGGAGACGAA-3′
Reverse: 5′-CCACACAAACACATCGAAGG-3′
DRD258°CNM_012547.1230Forward: 5′-CATTGTCTGGGTCCTGTCCT-3′
Reverse: 5′-GCTCTGAAAGCTCGACTGCT-3′
DRD359°CNM_017140.2104Forward: 5′-GAGCTCCTGTAGACGTGTGG-3′
Reverse: 5′-TGGGATCCCCTGTTGTGTTG-3′
DRD456°CNM_012944.2166Forward: 5′-GTAGTTGGGGCCTTCCTGTA-3′
Reverse: 5′-CGGCATTGAAGATGGTGTAG-3′
DRD558°CNM_012768.1146Forward: 5′-GTATCATCAGCGTGGACCGT-3′
Reverse: 5′-CCAATTGAGTTGGACCGGGA-3′
Actin56°CNM_031144.3165Forward: 5′-TGTCACCAACTGGGACGATA-3′
Reverse: 5′-GGGGTGTTGAAGGTCTCAAA-3′