Primers used for quantitative real-time PCR to assess mRNA expression of the five subtypes of dopamine receptors and β-actin
| Gene | Annealing temperature | Accession number | Product length (bp) | Primer sequences |
|---|---|---|---|---|
| DRD1 | 55°C | NM_012546.3 | 168 | Forward: 5′-TCCTTCAAGAGGGAGACGAA-3′ |
| Reverse: 5′-CCACACAAACACATCGAAGG-3′ | ||||
| DRD2 | 58°C | NM_012547.1 | 230 | Forward: 5′-CATTGTCTGGGTCCTGTCCT-3′ |
| Reverse: 5′-GCTCTGAAAGCTCGACTGCT-3′ | ||||
| DRD3 | 59°C | NM_017140.2 | 104 | Forward: 5′-GAGCTCCTGTAGACGTGTGG-3′ |
| Reverse: 5′-TGGGATCCCCTGTTGTGTTG-3′ | ||||
| DRD4 | 56°C | NM_012944.2 | 166 | Forward: 5′-GTAGTTGGGGCCTTCCTGTA-3′ |
| Reverse: 5′-CGGCATTGAAGATGGTGTAG-3′ | ||||
| DRD5 | 58°C | NM_012768.1 | 146 | Forward: 5′-GTATCATCAGCGTGGACCGT-3′ |
| Reverse: 5′-CCAATTGAGTTGGACCGGGA-3′ | ||||
| Actin | 56°C | NM_031144.3 | 165 | Forward: 5′-TGTCACCAACTGGGACGATA-3′ |
| Reverse: 5′-GGGGTGTTGAAGGTCTCAAA-3′ |