List of antibodies used in this study
| Antibody | Origin | Catalog # | Company | Dilution (TBST) |
|---|---|---|---|---|
| Primary antibodies | ||||
| Anti-NeuN | Mouse | MAB377 | Chemicon | 1:400 |
| Anti-GFAP | Rabbit | G9269 | Sigma-Aldrich | 1:200 |
| Anti-S100β | Mouse | S2532 | Sigma-Aldrich | 1:200 |
| Anti-Iba1 | Rabbit | 019-19741 | Wako | 1:200 |
| Anti-Laminin | Rabbit | L9393 | Sigma-Aldrich | 1:200 |
| Anti-Desmin | Rabbit | ab8592 | Abcam | 1:200 |
| Anti-CD31 | Rat | 550274 | BD Biosciences | 1:200 |
| Anti-CD13 | Goat | AF2335 | R&D Systems | 1:200 |
| Anti-PDGFRβ | Goat | AF-1042 | R&D Systems | 1:100 |
| Anti-Aqp4 | Rabbit | AB3594 | Millipore | 1:200 |
| Anti-CD11b | Rat | MCA711G | AbD Serotec | 1:200 |
| Anti-αSMA | Mouse | M0851 | Dako | 1:200 |
| Anti-Bmal1 | Rabbit | sc-48790 | Santa Cruz Biotechnology | 1:200 |
| Secondary antibody | ||||
| Alexa Fluor 488-conjugated goat anti-mouse IgG | A-11001 | Invitrogen | 1:400 | |
| Alexa Fluor 546-conjugated goat anti-mouse IgG | A-11030 | Invitrogen | 1:400 | |
| Alexa Fluor 546-conjugated goat anti-rabbit IgG | A-11035 | Invitrogen | 1:400 | |
| Alexa Fluor 633-conjugated goat anti-rabbit IgG | A-21070 | Invitrogen | 1:400 | |
| Alexa Fluor 488-conjugated donkey anti-goat IgG | A-11055 | Invitrogen | 1:400 | |
| Alexa Fluor 546-conjugated donkey anti-goat IgG | A-11056 | Invitrogen | 1:400 | |
| Alexa Fluor 488-conjugated goat anti-rat IgG | A-11006 | Invitrogen | 1:400 | |
| Alexa Fluor 546-conjugated goat anti-rat IgG | A-11081 | Invitrogen | 1:400 |