Antibodies used in this study
| Antibody name | Specificity | Host species | Dilutions | Source |
|---|---|---|---|---|
| T49 | Mouse tau | Mouse monoclonal | 1:1000 (ICC, WB) | In-house (Kosik et al., 1988) |
| R2295 mTau | Mouse tau | Rabbit polyclonal | 1:1000 (ICC, WB) | (Guo et al., 2016a) |
| AT8 | Tau phosphorylation at Ser202 and Thr 205 | Mouse monoclonal | 1:10,000 (IHC), 1:5000 (IF), 1:2000 (EM) | ThermoFisher Scientific (RRID:AB_223647) |
| 17025 | Raised against recombinant human tau | Rabbit polyclonal | 1:1000 | In-house (Ishihara et al., 1999; RRID:AB_2315435) |
| Tau5 | Tau (aa 210–230) | Mouse monoclonal | 0.05 μg/μl (IP), 2.5 μg/ml as capture antibody in tau ELISA | Gift from Dr. Lester Binder (RRID:AB_663326) |
| PHF-1 | Tau phosphorylation at Ser396 and Ser404 | Mouse monoclonal | 1:1000 (WB), 1:2000 (IHC) | Gift from Dr. Peter Davies (Greenberg et al., 1992; RRID:AB_2313687) |
| Anti-4R tau | 4R tau | Rabbit polyclonal | 1:5000 | CosmoBio |
| RD3 | 3R tau | Mouse monoclonal | 1:1000 | Millipore (RRID:AB_310013) |
| MC1 | Tau in the pathological conformation | Mouse monoclonal | 1:1000 (IHC) | Gift from Dr. Peter Davies (Jicha et al., 1997a; RRID:AB_2314773) |
| AT180 | Tau phosphorylation at Thr231 | Mouse monoclonal | 1:1000 (IHC) | Thermo Fisher (RRID:AB_223649) |
| TG3 | Conformational specific tau phosphorylation at Thr231 | Mouse monoclonal | 1:250 (IHC) | Gift from Dr. Peter Davies (Jicha et al., 1997b) |
| GAPDH (6C5) | Glyceraldehyde-3-phosphate dehydrogenase | Mouse monoclonal | 1:3000 | Advanced Immunochemical |
| GFAP | Glial fibrillary acidic protein (astrocytes) | Rat polyclonal | 1:1000 (IF) | In-house (Lee et al., 1984) |
| Olig-2 | Oligodendrocyte marker | Rabbit polyclonal | 1:500 (IF) | Millipore (RRID:AB_570666) |
| 17028 | MAP2 | Rabbit polyclonal | 1:5000 (ICC) | In-house (Volpicelli-Daley et al., 2011) |
Description of all antibodies used in this study, including specific proteins/epitopes they recognize, concentrations used, and how they were procured.