Detailed information of statistical analyses
| Figures | Sample size (n) | Statistical methods | p value, t value, and df |
|---|---|---|---|
| 1G | n = 3 independent experiments at each time point | One-way ANOVA (p = 0.0062, F(3,8) = 8.193) with Tukey's post test | OPCs versus D1 OLs, **p < 0.01 |
| OPCs versus D2 OLs, *p < 0.05 | |||
| OPCs versus D4 OLs, p > 0.05 | |||
| 1K | n = 145 OPCs and 116 OLs from 3 mice at P8 | Two-tailed unpaired | P8, ***p < 0.0001 (t = 34.33, df = 259) |
| n = 125 OPCs and 101 OLs from 3 mice at P14 | Student's t test | P14, ***p < 0.0001 (t = 14.87, df = 124) | |
| 2D | n = 5 sox2fl/fl | Two-tailed unpaired | MBP, **p < 0.002 (t = 4.779, df = 7) |
| n = 4 Cnp-Cre, Sox2fl/fl | Student's t test | SMI312, p = 0.178 ( t = 1.497, df = 7) | |
| 3D | n = 6 Sox2fl/fl | Two-tailed unpaired | Olig2+CC1+, **p = 0.0012 (t = 4.879, df = 8) |
| n = 4 Cnp-Cre, Sox2fl/fl | Student's t test | Olig2+PDGFRa+, p = 0.2314 (t = 1.295, df = 8) | |
| 3E | n = 6 sox2fl/fl | Two-tailed unpaired | p = 0.0041 (t = 3.975, df = 8) |
| n = 4 Cnp-Cre, Sox2fl/fl | Student's t test | ||
| 4B | n = 6 Sox2fl/fl | Two-tailed unpaired | Olig2+CC1+, p = 0.3783 (t = 0.9326, df = 8) |
| n = 4 Cnp-Cre, Sox2fl/fl | Student's t test | ||
| 4D | n = 8 Sox2fl/fl | Two-tailed unpaired | Sox2, **p = 0.0055 (t = 3.5424, df = 10) |
| n = 4 Cnp-Cre, Sox2fl/fl | Student's t test | Mbp, p = 0.5097 (t = 0.6837, df = 10) | |
| Plp, p = 0.119 (t = 1.705, df = 10) | |||
| Mag, p = 0.6681 (t = 0.4417, df = 10) | |||
| Mobp, p = 0.3381 (t = 1.006, df = 10) | |||
| Sox10, p = 0.3774 (t = 0.9237, df = 10) | |||
| 4E | n = 3 Sox2fl/fl | Two-tailed unpaired | p < 0.0001 (t = 42.99, df = 5) |
| n = 4 Cnp-Cre, Sox2fl/fl | Student's t test | ||
| 5C | n = 7 Sox2fl/fl | Two-tailed unpaired | Sox10+PDGFRα+, ***p < 0.0001 (t = 6.838, df = 12) |
| (cell #) | n = 7 Pdgfra-CreERT2, Sox2fl/fl | Student's t test | Sox10+, ***p < 0.0001 (t = 7.854, df = 12) |
| 5C | n = 4 Sox2fl/fl | Two-tailed unpaired | Sox2, p = 0.0145 (t = 3.398, df = 6) |
| (mRNA) | n = 4 Pdgfra-CreERT2, Sox2fl/fl | Student's t test | Pdgfra, p = 0.0032 (t = 4.745, df = 6) |
| 5F | n = 3 Sox2fl/fl | Two-tailed unpaired | Sox10+, p = 0.0009 (t = 8.856, df = 4) |
| n = 3 Pdgfra-CreERT2, Sox2fl/fl | Student's t test | Sox10+PDGFRα+, p = 0.0007 (t = 8.856, df = 4) | |
| Sox10+CC1+, p = 0.0122 (t = 4.344, df = 4) | |||
| 5H | n = 6 Sox2fl/fl | Two-tailed unpaired Student's t test | Sox2, p = 0.0042 (t = 3, df = 10) |
| n = 6 Pdgfra-CreERT2, Sox2fl/fl | Plp-Exon3b, p = 0.0027 (t = 3.964, df = 10) | ||
| Mbp, p = 0.0002 (t = 5.638, df = 10) | |||
| Mog, p = 0.0272 (t = 2.585, df = 10) | |||
| Bcas1, ***p < 0.0001 (t = 10.02, df = 10) | |||
| Ugt8a, ***p < 0.0001 (t = 8.683, df = 10) | |||
| Bmp4, ***p < 0.0001 (t = 11.27, df = 10) | |||
| Qk, p = 0.0012 (t = 4.461, df = 10) | |||
| 5I | n = 7 Sox2fl/fl | Two-tailed unpaired | ***p < 0.0001 (t = 7.504, df = 12) |
| n = 7 Pdgfra-CreERT2, Sox2fl/fl | Student's t test | ||
| 6B (EdU) | n = 5 Sox2fl/fl | Two-tailed unpaired | EdU+Sox10+ cells, p = 0.0137 (t = 3.053, dt = 9) |
| n = 6 Pdgfra-CreERT2, Sox2fl/fl | Student's t test | ||
| 6B (Ki67) | n = 4 Sox2fl/fl | Two-tailed unpaired | Ki67+Sox10+ cells, ***p < 0.0001 (t = 7.544, df = 7) |
| n = 5 Pdgfra-CreERT2, Sox2fl/fl | Student's t test | ||
| 6D | n = 3 Sox2fl/fl | Two-tailed unpaired | Caspase3+Sox10+, p = 0.4683 (t = 0.8008, df = 4) |
| n = 3 Pdgfra-CreERT2, Sox2fl/fl | Student's t test | TUNEL+Sox10+, p = 0.1737 (t = 1.653, df = 4) | |
| 7A | n = 8 Sox2fl/fl | Two-tailed unpaired | ***p < 0.0001 (t = 7.163, df = 10) |
| n = 4 Sox10-Cre, Sox2fl/fl | Student's t test | ||
| 7C | n = 5 Sox2fl/fl | Two-tailed unpaired | MBP, p = 0.0059 (t = 3.904, df = 7) |
| n = 4 Sox10-Cre, Sox2fl/fl | Student's t test | SMI312, p = 0.6733 (t = 0.4398, df = 7) | |
| 7D | n = 3 Sox2fl/fl | Two-tailed unpaired | Sox2, ***p < 0.0001 (t = 18.73, df = 5) |
| n = 4 Sox10-Cre, Sox2fl/fl | Student's t test | Sox3, p = 0.9522 (t = 0.06302, df = 5) | |
| MBP, p = 0.0098 (t = 4.052, df = 5) | |||
| CNP, p = 0.0072 (t = 4.378, df = 5) | |||
| MAG, p = 0.0278 (t = 3.070, df = 5) | |||
| 7E | n = 6 Sox2fl/fl | Two-tailed unpaired | Sox2, p = 0.0116 (t = 3.157, df = 9) |
| n = 4 Sox10-Cre, Sox2fl/fl | Student's t test | Mbp, p = 0.0093 (t = 3.298, df = 9) | |
| Plp-E3b, p = 0.0404 (t = 2.392, df = 9) | |||
| 7G (OPCs) | n = 7 Sox2fl/fl | OPCs: one-way ANOVA (***p < 0.0001, F(2,13) = 24.87) with Tukey's post test | Sox2fl/fl versus Sox10-Cre, Sox2fl/+, p > 0.05 |
| n = 5 Sox10-Cre, Sox2fl/+ | Sox2fl/fl versus Sox10-Cre, Sox2fl/fl, ***p < 0.0001 | ||
| n = 4 Sox10-Cre, Sox2fl/fl | Sox10-Cre, Sox2fl/+ versus Sox10-Cre, Sox2fl/fl, ***p < 0.001 | ||
| 7G (OLs) | n = 7 Sox2fl/fl | OLs: one-way ANOVA (***p = 0.0003, F(2,13) = 15.84) with Tukey's post test | Sox2fl/fl versus Sox10-Cre, Sox2fl/+, p > 0.05; Sox2fl/fl versus Sox10-Cre, Sox2fl/fl, ***p < 0.0001 |
| n = 5 Sox10-Cre, Sox2fl/+ | Sox10-Cre, Sox2fl/+ versus Sox10-Cre, Sox2fl/fl, **p < 0.01 | ||
| n = 4 Sox10-Cre, Sox2fl/fl | |||
| 7I | n = 6 Sox2fl/fl | Two-tailed unpaired | PDGFRα+ OPCs, ***p < 0.0001 (t = 6.392, df = 8) |
| n = 4 Sox10-Cre, Sox2fl/fl | Student's t test | CC1+ OLs, **p < 0.001 (t = 4.848, df = 8) | |
| 8D | n = 89 OPCs and 80 OLs from 3 mice | Two-tailed unpaired Student's t test | ***p < 0.0001 (t = 25.89, df = 167) |
| 8G | n = 4 CFA | Two-tailed unpaired | ***p < 0.0001 (t = 17.9, df = 6) |
| n = 4 MOG | Student's t test | ||
| 8I | n = 99 OPCs and 62 OLs from 3 mice | Two-tailed unpaired | ***p < 0.0001 (t = 20.67, df = 159) |
| Student's t test | |||
| 9B3 | n = 3 Sox2fl/fl | Two-tailed unpaired | Sox2, p = 0.0262 (t = 3, df = 4) |
| n = 3 Pdgfra-CreERT2, Sox2fl/fl | Student's t test | ||
| 9D | n = 3 Sox2fl/fl | Two-tailed unpaired | CC1+ OLs, p = 0.0018 (t = 7.382, df = 4) |
| n = 3 Pdgfra-CreERT2, Sox2fl/fl | Student's t test | CC1+BrdU+ OLs, p = 0.0175 (t = 3.904, df = 4) | |
| PDGFRα+ OPCs, p = 0.0100 (t = 4.608, df = 4) | |||
| PDGFRα+Ki67+ OPCs, p = 0.0007 (t = 9.604, df = 4) | |||
| 9F | n = 3 Sox2 WT + Cuprizone | One-way ANOVA (***p < 0.0001, F(2,6) = 100.2) with Tukey's post test | Sox2 WT + Cup versus Sox2 cKO + Cup, ***p < 0.001 |
| n = 3 Sox2 cKO + Cuprizone | Sox2 WT + Cup versus Sox2 WT + normal diet, ***p < 0.001 | ||
| n = 4 Sox2 WT + normal diet | Sox2 cKO + Cup versus Sox2 WT + normal diet, p > 0.05 | ||
| 9H | n = 3 Sox2 WT | Two-tailed unpaired | MBP, p = 0.0287 (t = 3.346, df = 4) |
| n = 3 Sox2 cKO | Student's t test | SMI312, p = 0.4334 (t = 0.9699, df = 4) | |
| 10C | n = 3 Sox2 WT + MOG | One-way ANOVA (***p = 0.0009, F(2,6) = 27.90) with Tukey's post test | Sox2 WT + MOG versus Sox2 cKO + MOG, **p < 0.01 |
| n = 3 Sox2 cKO + MOG | Sox2 WT + MOG versus Sox2 WT + CFA, ***p < 0.001 | ||
| n = 3 Sox2 WT + CFA | Sox2 cKO + MOG versus Sox2 WT + CFA, p > 0.05 | ||
| 10E | n = 3 Sox2 WT + MOG | Two-tailed unpaired | p = 0.0019 (t = 7.303, df = 4) |
| n = 3 Sox2 cKO + MOG | Student's t test | ||
| 10F | n = 3 Sox2 WT + MOG | One-way ANOVA (***p < 0.0001, F(2,6) = 69.92) with Tukey's post test | Sox2 WT + MOG versus Sox2 cKO + MOG, ***p < 0.001 |
| n = 3 Sox2 cKO + MOG | Sox2 WT + MOG versus Sox2 WT + CFA, ***p < 0.001 | ||
| n = 3 Sox2 WT + CFA | Sox2 cKO + MOG versus Sox2 WT + CFA, p > 0.05 |