Neuronal types/conditions | Data | Statistical method | Df | Sum Sq | Mean Sq | F-value | p-value | Significance | ||
---|---|---|---|---|---|---|---|---|---|---|
Figure 1C,D | JO-B vs AMMC-B1 | Integrated Ca2+ response | Two-way ANOVA with repeated measures | Strain | 1 | 145 | 145 | 34.7 | 2.98E-05 | *** |
Residuals | 15 | 62.9 | 4.19 | |||||||
IPI | 9 | 6.56 | 0.73 | 2.68 | 6.85E-03 | ** | ||||
Interaction | 9 | 12.0 | 1.33 | 4.89 | 1.12E-05 | *** | ||||
Residuals | 135 | 36.8 | 0.273 | |||||||
Figure 2C | Rdl-RNAi vs Control | Integrated Ca2+ response | Two-way ANOVA with repeated measures | Strain | 1 | 53.4 | 53.4 | 8.6 | 8.80E-03 | ** |
Residuals | 18 | 111 | 6.19 | |||||||
IPI | 9 | 25.3 | 2.81 | 14.7 | <2E-16 | *** | ||||
Interaction | 9 | 10.91 | 1.21 | 6.34 | 1.11E-07 | *** | ||||
Residuals | 162 | 31.0 | 0.191 | |||||||
Figure 5B | TNT vs IMPTNT | Integrated Ca2+ response | Two-way ANOVA with repeated measures | Strain | 1 | 1.03 | 1.03 | 0.318 | 0.578 | |
Residuals | 24 | 77.7 | 3.24 | |||||||
IPI | 9 | 1.69 | 0.187 | 4.77 | 8.43E-06 | *** | ||||
Interaction | 9 | 0.920 | 0.102 | 2.60 | 7.19E-03 | ** | ||||
Residuals | 216 | 8.48 | 0.0393 | |||||||
Figure 5C | TNT vs IMPTNT | Integrated Ca2+ response | Two-way ANOVA with repeated measures | Strain | 1 | 0.0100 | 9.40E-03 | 4.00E-03 | 0.949 | |
Residuals | 25 | 56.4 | 2.26 | |||||||
IPI | 9 | 3.34 | 0.371 | 4.35 | 3.08E-05 | *** | ||||
Interaction | 9 | 1.61 | 0.179 | 2.10 | 0.0308 | * | ||||
Residuals | 225 | 19.2 | 0.0854 | |||||||
Figure 5D | TNT vs IMPTNT | Integrated Ca2+ response | Two-way ANOVA with repeated measures | Strain | 1 | 0.510 | 0.506 | 0.210 | 0.651 | |
Residuals | 24 | 57.9 | 2.41 | |||||||
IPI | 9 | 1.62 | 0.180 | 3.83 | 1.65E-04 | *** | ||||
Interaction | 9 | 0.503 | 0.0559 | 1.19 | 0.305 | |||||
Residuals | 216 | 10.2 | 0.0471 |
Data | Statistical method | IPI | Sample size | U | p-value | Adjusted p-value | ||||
---|---|---|---|---|---|---|---|---|---|---|
Figure 2B | Rdl-RNAi vs Control | Integrated Ca2+ response (Raw df/F) | Mann–Whitney U test followed by Bonferroni multiple comparisons | 15 | 9, 11 | 4 | 5.47E-04 | 5.47E-03 | ** | |
25 | 9, 11 | 8 | 1.62E-03 | 0.0162 | * | |||||
35 | 9, 11 | 9 | 2.09E-03 | 0.0209 | * | |||||
45 | 9, 11 | 17 | 0.0135 | 0.135 | ||||||
55 | 9, 11 | 17 | 0.0135 | 0.135 | ||||||
65 | 9, 11 | 31 | 0.160 | 1.00 | ||||||
75 | 9, 11 | 22 | 0.0367 | 0.367 | ||||||
85 | 9, 11 | 34 | 0.239 | 1.00 | ||||||
95 | 9, 11 | 33 | 0.210 | 1.00 | ||||||
105 | 9, 11 | 32 | 0.184 | 1.00 | ||||||
Peak Ca2+ response (Raw df/F) | Mann–Whitney U test followed by Bonferroni multiple comparisons | 15 | 9, 11 | 2 | 3.08E-04 | 3.08E-03 | ** | |||
25 | 9, 11 | 7 | 1.24E-03 | 0.0124 | * | |||||
35 | 9, 11 | 7 | 1.24E-03 | 0.0124 | * | |||||
45 | 9, 11 | 11 | 3.44E-03 | 0.0344 | * | |||||
55 | 9, 11 | 15 | 8.76E-03 | 0.0876 | ||||||
65 | 9, 11 | 24 | 0.0527 | 0.527 | ||||||
75 | 9, 11 | 16 | 0.0109 | 0.109 | ||||||
85 | 9, 11 | 35 | 0.271 | 1.00 | ||||||
95 | 9, 11 | 33 | 0.210 | 1.00 | ||||||
105 | 9, 11 | 33 | 0.210 | 1.00 |
↵Asterisks indicate statistical significance: ***p < 0.001, **p < 0.01, *p < 0.05.
Df, Degree of freedom; Sq, square.