Primer sequences used for genotyping, qRT-PCR, and ISH probe synthesis
| Gene (purpose) | Forward sequence | Reverse sequence |
|---|---|---|
| Ctcf (genotype) | CTAGGAGTGTAGTTCAGTGAGGCC | GCTCTAAAGAAGGTTGTGAGTTC |
| Sry (genotype) | GCAGGTGGAAAAGCCTTACA | AAGCTTTGCTGGTTTTTGGA |
| Cre (genotype) | TGACCAGAGTCATCCTTAGCG | AATGCTTCTGTCCGTTTGCC |
| Puma (genotype) | AGGCTGTCCCTGCGGTCATCC | WT: GGACTGTCGCGGGCTAGACCCTCTA |
| MUT: ACCGCGGGCTCCGAGTAGC | ||
| Rosa mTmG (genotype) | CTCTGCTGCCTCCTGGCTTCT | WT: CGAGGCGGATCACAAGCAATA |
| MUT: TCAATGGGCGGGGGTCGTT | ||
| Ctcf (qRT-PCR) | CGATATGCTCTCATCCAGCA | TCCCACACTTGGAACAGACA |
| Lhx6 (qRT-PCR) | CTTGGGCTGACTGTCCTGTT | GGTGCGGCAGACAAATCTAT |
| B actin (qRT-PCR) | CTGTCGAGTCGCGTCCACCC | ACATGCCGGAGCCGTTGTCG |
| Lhx8 (qRT-PCR) | CCACCCATGTTGGAAGAAAT | CATTGGATGGGGTAACAAGG |
| Cxcr4 (qRT-PCR) | GAAGTAGATGGTGGGCAGGA | ACGGCTGTAGAGCGAGTGTT |
| Sst (qRT-PCR) | GGGCATCATTCTCTGTCTGG | GAGGCAAGGAAGATGCTGTC |
| Lhx8 (probe) | GAAGAGCGATCAGATGTTTGTG | TAATACGACTCACTATAGGGCACCTGTATGACACGTCTGCTT |
| Pvalb (probe) | GGATGTCGATGACAGACGTG | TAATACGACTCACTATAGGGACTCAACCCCTTCCCTTCC |
| Sst (probe) | TGAAGGAGACGCTACCGAAG | TAATACGACTCACTATAGGGAGGGTCAAGTTGAGCATCG |
| Gbx2 (probe) | GATGAAGAGAAGCTAGAGCCCC | TAATACGACTCACTATAGGGAGGTTCAGATCCTGTGACTTCC |
| Gad1 (probe) | GGGACCCTTGAACCGTAGAG | TAATACGACTCACTATAGGGCCCGTACTTCAGGGTGTCTC |
| Cr (probe) | GATGCTGACGGAAATGGG | TAATACGACTCACTATAGGGCCCTACCAGCCACCCTCT |
| VIP (probe) | CCTGGCATTCCTGATACTCTTC | TAATACGACTCACTATAGGGATTCTCTGATTTCAGCTCTGCC |