Summary of all animals used in this studya
| Experiment | No. of mice | No. of recorded neurons |
|---|---|---|
| ABR recordings | 12 | NA |
| Retrograde tracing for quantification of layer 5 versus layer 6 distribution | 10 | NA |
| Laser uncaging of glutamate for mapping of inputs | 7 | 8 cells per layer |
| Double injection of retrobeads into IC and AAV-ChR2 into MGB for stimulation of thalamocortical terminals | 5 | Without TTX: 5 cells per layer |
| With TTX: 11 cells in layer 5 and 13 cells in layer 6 | ||
| Triple injection of retrograde AAV virus into IC, AAV-mCherry into MGB, and Cre-dependent AAV in AC | 2 | NA |
↵aAll animals are BALB/C, 2–3 months old, both sexes.