Table 2.

Rating of behavioral signs of withdrawal

CTRLEtOH-WDL 12 hEtOH-WDL 48 hEtOH-WDL 14 d
MedianIQRMedianIQRpMedianIQRpMedianIQRp
Global rating
    Vehicle0.00.0–0.03.03.0–4.5***2.01.0–2.0*0.00.0–0.5n.s.
    l-DOPA0.00.0–0.02.01.0–2.0∧∧1.00.5–1.0n. s.0.00.0–0.5n.s.
Individual signs
    Body tremors
        Vehicle0.00.0–0.00.50.0–1.0*0.00.0–0.5n. s.0.00.0–0.0n.s.
        l-DOPA0.00.0–0.00.00.0–1.0n. s.0.00.0–0.0n. s.0.00.0–0.0n.s.
    Tail stiffness
        Vehicle0.00.0–0.01.01.0–2.0**1.00.5–2.0* *0.00.0–0.0n.s.
        l-DOPA0.00.0–0.00.50.0–1.0n. s.0.00.0–0.0n. s.0.00.0–0.0n.s.
    Irritability to touch
        Vehicle0.00.0–0.02.01.0–2.0***0.50.0–0.5n. s.0.00.0–0.5n.s.
        l-DOPA0.00.0–0.01.00.5–1.0∧1.00.0–1.0n. s.0.00.0–0.5n.s.

  • Each individual sign of withdrawal was rated on a 0–2 point scale, with 0 representing absence of sign, 1 representing moderate severity, and 2 representing extreme severity. Individual withdrawal sign ratings were then combined to produce a global score. Data are indicated as median and interquartile range (IQR) of n = 8 rats.

  • ↵***p < 0.001,

  • ↵**p < 0.01,

  • ↵*p < 0.05 Dunn's post hoc test versus CTRL;

  • ↵∧∧p < 0.01,

  • ↵∧p < 0.05 two-tailed Mann–Whitney U test versus vehicle.