Table 2.

Properties of identified low-rhythmic neurons

Cell nameMS13cMS109oMS83dMS68aTV78lMS16dMS09TV77qMS53TV68aMS10MS86bMS33aMS104e
Recoveryabcca
Target hemisphereLeftLeftRightLeftLeftLeftRightLeftLeftRightLeftLeftRightRight
Proportion of DG terminals∼99%uu∼90%98%u62%30%∼20%21%uuu0%
Innervated areasDG, uuuDG, CA3DG, CA3DG, uDG, CA3CA3, DGCA3, DG, CA2, CA1, SUB, MSCA3, DG, CA2, CA1, SUB, MSCA3, DG, uuCA3, CA1, SUBdCA1, RSg, SUBd, PrSd
Immunoreactivity
    PVaaxatadsaatadsadads+a+axd+
    Calbindina+ads+xa+daaadadaaxxas
    SATB1unxnn+uun+un+unxn+
    mGluR1ad+xxdds+uuds+d+ds+uds+xds+
    VGATxuuut+ut+t+xt+xuut+
    VGLUT2uuuututuuuuuuu
    VACHTuuuututuuuuuuu
    NK1Rduuuduudsd+s+uuuu
    PCP4duuud+uuds+uuuuux
    Calretininduuutaduuaduauuuu
    ChATauuuuauaaduuuxu
    Labeling strength+++++++++++++++++++++++++
Firing patterns
    Rhythmicity index M0.060.030.040.14(low)0.01u0.03u0.040.040.020.040.02
    Firing rate M (Hz)52 ± 950 ± 741 ± 824 ± 523 ± 1131 ± 6u31 ± 13u11 ± 1019 ± 918 ± 1025 ± 854 ± 16
    Firing rate IM (Hz)54 ± 1061 ± 1338 ± 1227 ± 922 ± 636 ± 10u16 ± 8u24 ± 1215 ± 716 ± 1114 ± 631 ± 19
    Burst incidence M (Hz, 20)4.64.92.61.00.31u1.5u0.51.80.40.34.7
    Burst incidence IM (Hz, 20)4.54.72.42.00.52.3u0.9u1.40.50.40.12.2
    Burst incidence M (Hz, 40)3.13.54.12.61.63u2.2u10.81.10.72.0
    Burst incidence IM (Hz, 40)2.72.23.52.761.83.2u1.5u2.31.11.10.52.2
    Interburst interval M226, 251222, 206206, 150273, 254537, 413252, 194u197, 207u417, 1282326, 347778, 807272, 190362, 364
    Interburst interval IM297, 244338, 379246, 160312, 220394, 394266, 196u446, 616u309, 275588, 949563, 963854, 1613301, 306
    Intraburst frequency M74 ± 1768 ± 1474 ± 1988 ± 3568 ± 2765 ± 18u81 ± 26u89 ± 3075 ± 2749 ± 860 ± 1472 ± 16
    Intraburst frequency IM73 ± 1574 ± 2281 ± 2989 ± 2564 ± 1672 ± 20u84 ± 29u71 ± 1981 ± 3154 ± 1358 ± 1477 ± 25
CA1d SWRs (130–230 Hz)
    n SWRs4033133561149u50u2745133279
    Mean rate inside (Hz)21.7147.44u15.160.659.97u6.2u1.7310.27u24.7815.61
    Lambda rate outside (Hz)53.8161.54u26.8421.8236.66u15.83u24.4515.16u8.1931.51
    SWR index−0.43−0.13u−0.28−0.94−0.57u−0.44u−0.87−0.19u0.5−0.34
    Poisson P-value<0.00010.01u0.005<0.0001<0.0001u0.003u<0.00010.05u<0.00010.001
    Mean rate before ripple (Hz)55.5062.27u34.7122.1333.32u12.41u26.3013.22u20.1637.30
    Mean rate after ripple (Hz)59.6365.00u50.0030.0846.81u46.11u36.8523.22u21.2545.53
    Rebound index0.040.02u0.180.150.17u0.58u0.170.27u0.030.10
    P-value (KS-test)0.090.54u0.0020.002<0.0001u<0.0001u0.13<0.0001u0.14<0.0001
CA1d theta (5–12 Hz)
    Theta cycles41929244822030429u374u217229165120517
    Theta off %0%0%0.9%7,7%0%1,6%u14,9%u19,4%13,1%18%1,7%0,2%
    LFP locationSPSPSPSPSPSPuSPuSP/SRSPSPSPSP
    Preferred theta phase251°272°340°290°u355°u234°u79°320°u29°342°
    Mean vector length (r)0.20.160.190.36u0.04u0.3u0.220.21u0.210.15
    Rayleigh P-value<0.0001<0.0001<0.0001<0.00010.2820.03u<0.0001u<0.0001<0.00010.983<0.0001<0.0001
    Total n spikes2490178520815935051674u1206u5223702762173943
    Spikes per cycle8.16.95.83.13.24.3u3.1u2.32.91.82.98.2
  • Positive (+) or undetectable (−) immunoreactivity to a molecule observed within subcellular domain.

  • Firing rates are expressed as mean of 1 s bins ± s.d. M, movement (locomotion and/or small movements). IM, immobility. Bursts are defined as >3 spikes with ISIs <40 ms or <20 ms (see burst incidence). Interburst intervals are in ms (median, interquartile range). Intraburst frequency expressed as mean ± SD (Hz). Some parameters for neurons TV78l and TV77q have been previously reported (Viney et al., 2018). Additional tests: The dendrites of TV78l and TV68a were immunopositive for SMI32.

  • aSoma was not recovered.

  • bLabeled soma and dendrites could not be directly linked to the projection axon.

  • cFiring patterns could not be unequivocally matched to the labeled neuron.

  • RSg, Granular retrosplenial cortex; PrSd, dorsal presubiculum; SUBd, dorsal subiculum; s, soma; n, nucleus; d, proximal dendrite; a, axon; t, axon terminals; x, tested but inconclusive; u, unknown.