Table 2.

zSTIM1 is required for axon guidance in vivo^{^a}

		zSTIM1-CTRL	zSTIM1-KD
Position of CaP axon distal tip at 24 hpf	Horizontal myoseptum	0/32	4/78
	Ventral notochord	2/32	25/78
	Ventral muscle boundary	30/32	49/78
Position of CaP axon distal tip at 48 hpf	Ventral notochord	0/44	3/40
	Ventral muscle boundary	0/44	4/40
	Entered lateral surface of muscle	44/44	33/40

↵^azSTIM1-CTRL and zSTIM1-KD CaP axons were visualized in Gal4^s1020t/UAS:mCherry embryos, with axons scored on the position of the most distal aspect of the axon at 24 or 48 hpf. The number of axons located at each position is represented as a fraction of total number of CaP axons visualized (values represent the number of axons imaged in 27 zSTIM1-KD and 12 zSTIM1-CTRL embryos).