Table 1.

POMC neuron recording parameters are not affected by energy state or deletion of VGAT in the DMH

DMH VGAT WTDMH VGAT KO
Ad libitumO/N FastRe-fedAd libitumO/N FastRe-fed
Input resistance, MΩ809 ± 52797 ± 48758 ± 33735 ± 46776 ± 59728 ± 48
Membrane potential, mV−59 ± 2.3−62 ± 2.8−63 ± 2.5−60 ± 3.4−59 ± 5.6−58 ± 2.9
  • The input resistance measured from POMC cells was not altered following deletion of VGAT in the DMH (F(1,99) = 1.196, p = 0.28; two-way ANOVA) or by challenges to the animal's energy state (F(2,99) = 0.369, p = 0.69; two-way ANOVA). Deletion of VGAT in the DMH (F(1,99) = 0.282, p = 0.59; two-way ANOVA), or energy state challenges (F(2,99) = 0.016, p = 0.98; two-way ANOVA) did not affect the membrane potential of POMC cells. Data are presented as mean ± SEM.