Table 1.

Key resources, suppliers, and unique identifying information

Reagent or ResourceSourceIdentifier
Antibodies
    Rabbit polyclonal anti-DsRedClontech LaboratoriesCatalog #632496, RRID:AB_10013483
    Goat anti-rabbit polyclonal AlexaFluor 594ThermoFisher ScientificCatalog #A-11037, RRID:AB_2534095
Chemicals, peptides, and recombinant proteins
    Collagenase type PSigma-AldrichCatalog #11249002001
    OCT compoundTissue-TekCatalog #4583
    Trypsin-EDTA 0.05%Fisher ScientificCatalog #25300054
    Fluoromount-GThermoFisher ScientificCatalog #00-4958-02
    LamininSigma-AldrichCatalog #L2020–1MG
    MEMFisher ScientificCatalog #11098050
    Penicillin-streptomycinFisher ScientificCatalog #15-140-122
    MEM vitamin solutionThermoFisher ScientificCatalog #SH3059901
    B-27 supplementFisher ScientificCatalog #17504044
    Horse serum, heat inactivatedThermoFisher ScientificCatalog #26050070
    Fura-2, AMThermoFisher ScientificCatalog #F1221
    Pluronic F-127ThermoFisher ScientificCatalog #P3000MP
    Human NaV1.7 plasmidDr. Manu Ben-Johny
    DMEMThermoFisher ScientificCatalog #11995073
    Fetal bovine serumThermoFisher ScientificCatalog #A3840101
    TTXAbcamCatalog #ab120054
    AH-TTXR&D SystemsCatalog #6159/100U
    PF 05089771Tocris BioscienceCatalog #5931
    ICA 121431Tocris BioscienceCatalog #5066/10
    PN3aDeuis et al. (2017)
Critical commercial assays
    Plasmid Maxi kitQiagenCatalog #12162
    RNAscope Fluorescent Multiplex kitAdvanced Cell DiagnosticsCatalog #320850
Deposited data
    Source Data (freely available through Mendeley)This studyhttp://dx.doi.org/10.17632/bxsvmy2zh3.1
    Movie of calcium imaging experimentThis studyhttp://dx.doi.org/10.17632/bxsvmy2zh3.1
Experimental Models: Organisms/Strains
    Mouse: Slc17a8iCre: Tg(Slc17a8-icre)1Edw/SealJThe Jackson LaboratoryRRID:IMSR_JAX:018147
    Mouse: B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/JThe Jackson LaboratoryRRID:IMSR_JAX:007914
    Homo sapiens: embryonic kidney cells stably transfected with NaV1.1, β1 and β2 subunitsKahlig et al. (2010)
    Homo sapiens: embryonic kidney cells stably transfected with NaV1.6Dr. Lori Isom
Oligonucleotide
    Trpm8 probe channel 3Advanced Cell DiagnosticsCatalog #420451-C3
    Scn1a probe channel 2Advanced Cell DiagnosticsCatalog #434181-C2
    Scn8a probe channel 2Advanced Cell DiagnosticsCatalog #434191-C2
    Scn9a probe channel 2Advanced Cell DiagnosticsCatalog #313341-C2
    Scn10a probe channel 2Advanced Cell DiagnosticsCatalog #426011-C2
Software and Algorithms
    pClamp 10 Software SuiteMolecular Deviceshttps://www.moleculardevices.com
    Image JSchneider et al. (2012)https://imagej.nih.gov/ij/index.html
    MATLAB 2017bMathWorkshttps://www.mathworks.com
    Software, algorithm, custom (MATLAB)This studyhttps://github.com/thelumpkinlab/Calcium-Imaging-Analysis-Script
    MetaMorph v7.50.6Molecular Deviceshttps://www.moleculardevices.com
    MetaFluor v7.60.3Molecular Deviceshttps://www.moleculardevices.com
    Prism v7.0bGraphPadhttps://www.graphpad.com