Figure | Parameter | Groups | Statistical test | Outcome | p |
---|---|---|---|---|---|
5D | E12.5 phospho-Erk1/2+regions | A-P position vs genotype (E12.5 control and Sufu-cKO) | Repeated-measures two-way ANOVA | F position × genotype (3,24) = 3.329 | 0.0365 |
Control (n = 5) vs Sufu-cKO (n = 5) Position 1 | Holm-Sidak's multiple comparisons test | t = 3.685, df = 5.547 | 0.018 | ||
Control (n = 5) vs Sufu-cKO (n = 5) Position 2 | t = 6.861, df = 4.873 | 0.0033 | |||
Control (n = 5) vs Sufu-cKO (n = 5) Position 3 | t =8.112, df = 4.949 | 0.0019 | |||
Control (n = 5) vs Sufu-cKO (n = 5) Position 4 | t = 5.310, df = 4.296 | 0.0099 | |||
5E | E14.5 phospho-Erk1/2+regions | A-P position vs genotype (E14.5 control and Sufu-cKO) | Repeated-measures two-way ANOVA | F position vs genotype (3,19) = 4.684 | 0.013 |
Control (n = 4) vs Sufu-cKO (n = 3) Position 1 | Holm-Sidak's multiple comparisons test | t = 7.255, df = 27 | <0.0001 | ||
Control (n = 5) vs Sufu-cKO (n = 5) Position 2 | t = 5.452, df = 27 | <0.0001 | |||
Control (n = 5) vs Sufu-cKO (n = 5) Position 3 | t = 4.665, df = 27 | 0.0001 | |||
Control (n = 4) vs Sufu-cKO (n = 4) Position 4 | t = 3.67, df = 27 | 0.0011 | |||
6D | Tbr2+ cells | Treatment (n = 3 per condition) | One-way ANOVA | F treatment (5,12) = 14.15 | 0.0001 |
DMSO vs Fgf15 | Holm-Sidak's multiple comparisons test | t = 3.319, df = 12 | 0.0537 | ||
DMSO vs Shh | t = 6.515, df = 12 | 0.0004 | |||
DMSO vs Fgf15+Shh | t = 5.651, df = 12 | 0.0014 | |||
DMSO vs Cyclo | t = 0.5662, df = 12 | 0.6585 | |||
DMSO vs Cyclo+Fgf15 | t = 4.4, df = 12 | 0.0095 | |||
Fgf15 vs Shh | t = 3.196, df = 12 | 0.0599 | |||
Fgf15 vs Fgf15+Shh | t = 2.332, df = 12 | 0.2072 | |||
Fgf15 vs Cyclo | t = 2.753, df = 12 | 0.1163 | |||
Fgf15 vs Cyclo+Fgf15 | t = 1.081, df = 12 | 0.6585 | |||
Shh vs Fgf15+Shh | t = 0.8646, df = 12 | 0.6585 | |||
Shh vs Cyclo | t = 5.949, df = 12 | 0.0009 | |||
Shh vs Cyclo+Fgf15 | t = 2.115, df = 12 | 0.2503 | |||
Fgf15+Shh vs Cyclo | t = 5.085, df = 12 | 0.0032 | |||
Fgf15+Shh vs Cyclo+Fgf15 | t = 1.251, df = 12 | 0.6572 | |||
Cyclo vs Cyclo+Fgf15 | t = 3.834, df = 12 | 0.0235 | |||
7I | Tbr2+ cells | Treatment: DMSO (n = 6) vs FGF15 (n = 6) | Unpaired t test (two-tailed) | t = 2.805, df = 10 | 0.0186 |
↵aSummary of statistical analyses performed for Figures 5–7.