Cluster analysis | |||||
---|---|---|---|---|---|
Genotype | Au particles | Parameter | Real data | Simulated data | p value |
RIM-BP2 WT | AZ (5 nm) | # of clusters/AZ | 0.29 ± 0.03 | 0.14 ± 0.01 | 3.68e-16 |
# of particles/cluster | 3.99 ± 0.15 | 3.50 ± 0.09 | 8.57e-08 | ||
Area of cluster (nm2) | 663.30 ± 80.9 | 503.78 ± 45.30 | 0.007 | ||
CaV2.1 (10 nm) | # of clusters/AZ | 0.78 ± 0.04 | 0.48 ± 0.03 | 6.44e-29 | |
# of particles/cluster | 4.36 ± 0.13 | 3.79 ± 0.08 | 5.72e-13 | ||
Area of cluster (nm2) | 976.25 ± 88.99 | 695.77 ± 55.90 | 2.26e-08 | ||
RIM-BP2 KO | AZ (5 nm) | # of clusters/AZ | 0.08 ± 0.02 | 0.05 ± 0.01 | 0.015 |
# of particles/cluster | 4.33 ± 0.43 | 3.71 ± 0.33 | 0.006 | ||
Area of cluster (nm2) | 759.82 ± 186.11 | 542.89 ± 125.05 | 0.09 | ||
CaV2.1 (10 nm) | # of clusters/AZ | 0.38 ± 0.04 | 0.15 ± 0.02 | 6.13 e-13 | |
# of particles/cluster | 3.80 ± 0.16 | 3.35 ± 0.06 | 0.0003 | ||
Area of cluster (nm2) | 659.50 ± 95.37 | 438.90 ± 29.90 | 0.005 |
Data are presented as mean ± SEM. Real data were compared to 500 random simulations to confirm that the observed clusters are significantly different from chance (random) occurrence. Student's t test was used to determine the statistical significance. p < 0.05 set as threshold for statistical significance. Statistically significant differences shown in bold.