Reagents, resources
| Reagents | Source | Catalog number or website |
|---|---|---|
| HiScribe T7 High Yield RNA Synthesis Kit | New England Biolabs | E2040S |
| RNeasy Mini Kit | Qiagen | 74104 |
| Alt-R S.p. Cas9 Nuclease 3NLS | Integrated DNA Technologies | 1074182 |
| PfuUltra II Fusion HotStart DNA Polymerase | Agilent | 600670 |
| Phusion High-Fidelity DNA Polymerase | New England Biolabs | M0520S |
| Taq DNA Polymerase with Standard Taq Buffer | New England Biolabs | M0273X |
| Zen 2.1 (Blue edition) | Carl Zeiss Microscopy | https://www.zeiss.com |
| Fiji/ImageJ | (Schindelin et al., 2012) | https://fiji.sc/ |
| R | R Foundation for Statistical Computing | https://www.r-project.org/ |