Gene IDs, Cas9 gRNAs, genotyping primers
| Ensembl number | |
|---|---|
| Gene | |
| dlk/Map3k12 | ENSDARG00000103651 |
| lzk/Map3k13 | ENSDARG00000009493 |
| CRISPR/Cas9 gRNAs | |
| GTG GGT GGG CAG CGG CGC TC AGG | Deletion: gRNA for exon 2 to delete kinase domain of dlk, “-” strand |
| GCT GTG GGA GAT GCT GAC CG GGG | Deletion: gRNA for exon 5 to delete kinase domain of dlk, “-” strand |
| CCC CGG AGG TGC TGT CCT GGA C | Deletion: gRNA 1 for exon 1 to make a small deletion in lzk, “+” strand |
| CCT CAA GCG CTC CTG CCT CCT GC | Deletion: gRNA 2 for exon 1 to make a small deletion in lzk, “+” strand |
| Genotyping primers | |
| 5′ GC CAA CCC TGT GGA GAC TAA ACC 3′ | Forward primer for mutant band of dlk (exon 2) |
| 5′ CCA GCA CTG TCT GAG CAG GAT C 3′ | Reverse primer for mutant band of dlk (exon 5) |
| 5′ AAG GGT TAC GGT TGG GGT TAG G 3′ | Forward primer for wild-type band of dlk (exon 4) |
| 5′ GTT GCG CAG TGA TGT CTG TGA A 3′ | Reverse primer for wild-type band of dlk (exon 4) |
| 5′ TCC TCG TGT TCC TCC AAC A 3' | Forward primer for lzk (exon 1) |
| 5′ GCT GTA AGT GAT GGA GAG GCA T 3' | Reverse primer for lzk (exon 1) |