Table 1.

List of primers used in this study

Primer nameSequence 5′–3′Experiment
Hdac2_P14FATT TGG GAG AAG GCC AGT TTGenotyping Hdac2-flox
Hdac2 P15RAAT TTC ACA GCC CCA GCT AAGGenotyping Hdac2-flox
Hdac2_P2CGA AAT ACC TGG GTA GAT AAA GCGenotyping Hdac2-flox
Avl-Cre-1CCC TGT TCA CTG TGA GTA GGGenotyping Avl-Cre
Avl-Cre-2AGT ATC TGG TAG GTG CTT CCA GGenotyping Avl-Cre
Avl-Cre-3GCG ATC CCT GAA CAT GTC CAT CGenotyping Avl-Cre
Avl-icre-transgene forwardACC CCG ACT TTG TGA TGT TTCGenotyping Avl-CreERT2
Avl-icre-transgene reverseCTG CCT GTC CCT GAA CAT GGenotyping Avl-CreERT2
Avl-icre-internal positive control forwardCAC GTG GGC TCC AGC ATTGenotyping Avl-CreERT2
Avil-icre-internal positive control reverseTCA CCA GTC ATT TCT GCC TTT GGenotyping Avl-CreERT2
Cacna2d1-Primer ACAT GGG TGG ACA AGA TGC AAGGenotyping Cacna2d1 KO
Cacna2d1-Primer BCTG CAC GAG ACT AGT GAG ACGGenotyping Cacna2d1 KO
Cacna2d1-Primer CCAT TCT CAA GAC TGT AGG CAG AGGenotyping Cacna2d1 KO
Rat Hdac3 forwardCCC CAG ATT TCA CGC TCC ATRT-PCR
Rat Hdac3 reverseTGA ATC TGG ACA CTG GGT GRT-PCR
Rat Cacna2d1 forwardGGA CCT ATT CAG TGG ATG GCT TGRT-PCR
Rat Cacna2d1 reverseCCA TTG GTC TTC CCA GAA CAT CTA GART-PCR
Rat Hdac2 forwardGAT GAG GAT GGA GAA GAC CCT GRT-PCR
Rat Hdac2 reverseATG ATC AGC GAC ATT CCT ACG ACCRT-PCR
Rat Gapdh forwardCAT CCC AGA GCT GAA CGG GAA GRT-PCR
Rat Gapdh reverseGTC CTC AGT GTA GCC CAG GAT GCRT-PCR
Mouse Hdac2 forwardGCT GGA GGA CTA CAT CAT GCC ART-PCR
Mouse Hdac2 reverseACC ATC ACC ATG GTG GAT GTC TAT GRT-PCR
Mouse Cacna2d1 forwardGGG TGG ACA AGA TGC AAG AAG ACRT-PCR
Mouse Cacna2d1 reverseTGG CGT GCA TTG TTG GGC TCRT-PCR
Mouse Tuba1a forwardCCA CTA CAC CAT TGG CAA GGA GART-PCR
Mouse Tuba1a reverseGGA GGT GAA GCC AGA GCC AGTRT-PCR
Mouse Cacna2d1 ChIP promoter forwardCTG GCC GAG CTC TGT TTC CAC AGTChIP-PCR (71 to 164 bp)
Mouse Cacna2d1 ChIP promoter reverseGGG CGG ACT CTC TAG CGC GTG GCTChIP-PCR
Mouse Cacna2d1 ChIP UTR forwardGAC ATG GGA CTA ACG TAC TAT GCA AAGChIP-PCR (440,221 to 440,390 bp)
Mouse Cacna2d1 ChIP UTR reverseTGC TCT ACT TCA TGC AAT ACT TCA GTG CChIP-PCR
Mouse Gapdh ChIP promoter forwardTCT CAG GTT CCG AGG AGG GAT ACChIP-PCR (–53 to 85 bp)
Mouse Gapdh ChIP promoter reverseTTG AGA CTT ACA AAC ACT CTC CTG AGChIP-PCR
Rat Cacna2d1 ChIP promoter forwardCCT CTC CTC GGC GAC CGC AGA TAA AChIP-PCR (–32 to 60 bp)
Rat Cacna2d1 ChIP promoter reverseACT GTG GAA ACG GAG CTC GGC CAGChIP-PCR
Rat Gapdh ChIP forwardCAT TGC AGA GGA TGG TAG AGG AChIP-PCR (–120 to –23 bp)
Rat Gapdh ChIP reverseGGA GAA GAA AGT CAG ATT AGC GTG GChIP-PCR