Vh, △Vh, and pH50 of the hPAC Channel mutants
| Vh at pH 4.6 | Vh at pH 5.5 | △Vh (mean ± SEM) | pH50 at 200 mV | Patch numbers | |
|---|---|---|---|---|---|
| WT | 80.1 ± 1.1 | 225.4 ± 6.4 | 148.5 ± 5 | 5.16 ± 0.003 | 6 |
| D91N | 99.7 ± 0.8 | 202.8 ± 4.7 | 103.1 ± 5.6 | 5.63 ± 0.191 | 6 |
| E94Q | 85.1 ± 4.4 | 231.2 ± 10.5 | 146.1 ± 3.7 | 5.40 ± 0.029 | 6 |
| H98A | 89.1 ± 1.8 | 241.3 ± 5.4 | 152.2 ± 4.6 | 5.21 ± 0.048 | 6 |
| K106N | 105.9 ± 8.7 | 249.4 ± 7.9 | 143.5 ± 8.3 | 5.19 ± 0.005 | 6 |
| E107Q | 101.7 ± 6.1 | 199.2 ± 4.6 | 97.5 ± 0.9 | 5.71 ± 0.075 | 6 |
| E107A | 98.1 ± 3.6 | 169.8 ± 10.6 | 71.7 ± 8.9 | 5.70 ± 0.068 | 6 |
| E107C | 106.7 ± 6.9 | 214.3 ± 7.4 | 107.6 ± 10.2 | 5.38 ± 0.047 | 6 |
| E107R | 92.9 ± 7.6 | 156.2 ± 13.0 | 64.4 ± 8 | 6.02 ± 0.045 | 6 |
| V108A | 100.4 ± 9.7 | 208.8 ± 11.2 | 108.4 ± 10.1 | 5.23 ± 0.051 | 6 |
| D109N | 90.3 ± 4.7 | 250.3 ± 4.8 | 160.0 ± 5.9 | 5.19 ± 0.002 | 6 |
| D112N | 81.8 ± 7.8 | 225.0 ± 10.2 | 143.2 ± 14.8 | 5.43 ± 0.113 | 6 |
| H130/131A | 84.5 ± 6.2 | 239.2 ± 7.0 | 154.7 ± 9.7 | 5.32 ± 0.046 | 6 |
| E133Q | 105.7 ± 3.8 | 221.9 ± 6.4 | 116.2 ± 7.5 | 5.39 ± 0.055 | 6 |
| E176Q | 76.1 ± 4.7 | 212.0 ± 8.3 | 135.9 ± 14.8 | 5.26 ± 0.025 | 6 |
| R180Q | 77.5 ± 4.5 | 206.5 ± 8.8 | 128.9 ± 9.2 | 5.17 ± 0.019 | 6 |
| E181Q | 89.0 ± 0.1 | 230.4 ± 5.4 | 141.4 ± 5.9 | 5.16 ± 0.016 | 6 |
| D195N | 75.8 ± 5.5 | 224.0 ± 7.3 | 148.2 ± 2.3 | 5.38 ± 0.038 | 6 |
| E209Q | 80.3 ± 11.6 | 221.8 ± 12.5 | 141.5 ± 13.7 | 5.18 ± 0.005 | 6 |
| E224Q | 110.7 ± 8.0 | 239.5 ± 9.6 | 128.8 ± 13.3 | 5.38 ± 0.038 | 6 |
| E250Q | 104.2 ± 9.1 | 268.8 ± 7.9 | 164.6 ± 15.8 | 5.18 ± 0.005 | 6 |
| E254Q | 86.0 ± 4.9 | 275.8 ± 6.6 | 189.8 ± 3.7 | 5.16 ± 0.007 | 6 |
| K241N | 87.5 ± 9.7 | 195.6 ± 14.3 | 107.6 ± 13.2 | 5.15 ± 0.007 | 6 |
| E257Q | 294.6 ± 5.4 | 334.8 ± 6.8 | 40.2 ± 1.2 | / | 6 |
| E257A | 277.6 ± 9.4 | 313.5 ± 7.9 | 35.9 ± 2.3 | / | 6 |
| E257C | 288.2 ± 11.8 | 308.3 ± 11.2 | 21.7 ± 2.4 | / | 6 |
| E257D | 275.3 ± 12.5 | 306.4 ± 14.5 | 31.4 ± 2.2 | / | 6 |
| R259Q | 123.4 ± 7.6 | 205.8 ± 7.2 | 82.4 ± 7.3 | 5.42 ± 0.019 | 6 |
| E261Q | 103.6 ± 10.9 | 244.1 ± 19.3 | 140.5 ± 21.9 | 5.34 ± 0.059 | 6 |
| D269N | 88.5 ± 2.4 | 267.5 ± 3.5 | 179.0 ± 12.2 | 5.27 ± 0.071 | 6 |
| K288N | 144.2 ± 11.7 | 251.2 ± 12.8 | 107.6 ± 13.2 | 5.64 ± 0.045 | 6 |
| D289N | 306.0 ± 14.7 | 317.6 ± 6.9 | 11.6 ± 3.9 | / | 6 |
| D289K | 262.1 ± 11.7 | 305.1 ± 9.3 | 43.1 ± 2.9 | / | 6 |
| D289E | 244.3 ± 13.2 | 282.8 ± 10.5 | 38.5 ± 3.1 | / | 6 |
| D289A | 274.6 ± 15.1 | 313.0 ± 10.7 | 39.4 ± 2.5 | / | 6 |
| D297N | 93.2 ± 3.6 | 280.2 ± 5.4 | 187.0 ± 2.2 | 5.32 ± 0.119 | 6 |
| P290A | 96.2 ± 11.8 | 215.7 ± 16.5 | 119.7 ± 15.2 | 5.24 ± 0.023 | 6 |
| Uninjected oocytes | 293.3 ± 18.9 | 346.7 ± 22.5 | 53.4 ± 3.2 | / | 6 |