Table 3.

NMDG-sensitive Na+ leak currents and firing properties of dTomato-positive control and cNalcnKO SCN neurons during the day and at night

Firing rate (Hz)Vm (mV)INaL (pA/pF)Rin (GΩ)
DayControl3.5 ± 0.3a,e
n = 20
−40.6 ± 0.6b,f
n = 16
2.0 ± 0.2c
n = 25
2.4 ± 0.2d,g
n = 22
cNalcnKO1.8 ± 0.2a
n = 27
−46.9 ± 1.2b
n = 21
1.0 ± 0.1c
n = 28
3.9 ± 0.3d,j
n = 22
NightControl1.7 ± 0.2e
n = 19
−45.6 ± 0.9f
n = 19
2.0 ± 0.2h
n = 16
1.4 ± 0.1g,i
n = 16
cNalcnKO1.7 ± 0.2
n = 32
−46.4 ± 1.4
n = 15
1.0 ± 0.1h
n = 13
2.6 ± 0.2i,j
n = 13
  • Values are mean ± SEM; n = number of cells. Vm, membrane potential.

  • a-dValues in daytime dTomato+ control and daytime dTomato+ cNalcnKO SCN neurons are significantly different (a,c,dp < 0.0001; bp < 0.001; one-way ANOVA with Tukey's post hoc test).

  • e-gValues in daytime and nighttime dTomato+ control SCN neurons are significantly different (ep < 0.0001; fp < 0.01; gp < 0.05; one-way ANOVA with Tukey's post hoc test).

  • h,iValues in nighttime dTomato+ control and nighttime dTomato+ cNalcnKO SCN neurons are significantly different (hp < 0.001; ip < 0.05; one-way ANOVA with Tukey's post hoc test).

  • jValues in daytime and nighttime dTomato+ cNalcnKO SCN neurons are significantly different (jp < 0.01; one-way ANOVA with Tukey's post hoc test).