Table 1.

Summary of different DCV cargo properties

CargoPersistent events (%)kDa*StimulationStudy
SemapHluorin36 ± 690–13060 mm KClDe Wit et al. (2009)
SemapHluorin38.2 ± 6.890–13016 × 50 APs at 50 HzDe Wit et al. (2009)
BDNF-pHluorin26.5 ± 7.258–4260 mm KClDe Wit et al. (2009)
tPA-SpH22 ± 59760 mm KClDe Wit et al. (2009)
NPY-pHluorin7 ± 334–4260 mm KClDe Wit et al. (2009)
SemaΔBD-pHluorin#5.8 ± 5.280–12060 mm KClDe Wit et al. (2009)
NPY-pHluorin7.7 (median)34–4216 × 50 APs at 50 HzThis study, Figure 2
NPY-pHluorin0 (median)34–42TBSThis study, Figure 2
BDNF-pHluorin60.75 (median)58–4216 × 50 APs at 50 HzThis study, Figure 2
BDNF-pHluorin46.3 (median)58–42TBSThis study, Figure 2
  • De Wit et al. (2009).

  • *kDa depends on degree of peptide processing.

  • #SemaΔBD-pHluorin: Semaphorin3A lacking C-terminal polybasic domain that interacts with luminal glycoproteins.

  • Persistent events are characterized by an abrupt increase in fluorescence that remained elevated above baseline (30 s in 80% of events).

  • Persistent events are plotted as mean ± SEM.

  • TBS consists of 5 bursts of 4 APs at 100Hz with 200-ms interval repeated three to four times.