Mechanism of Stimulation of Glucose Transport by H2O2: Role of Phospholipase C

doi:10.1006/abbi.1998.1026

Archives of Biochemistry and Biophysics

Volume 362, Issue 1, 1 February 1999, Pages 113-122

https://doi.org/10.1006/abbi.1998.1026 Get rights and content

Abstract

Exposure of Clone 9 cells, a rat liver cell line, to hydrogen peroxide (H₂O₂) resulted in a striking and rapid stimulation of glucose transport (8- to 10-fold in 1 h). A comparable response was found in 3T3-L1 preadipocytes, C₂C₁₂myoblasts, and NIH 3T3 fibroblasts, which, similar to Clone 9 cells, express only the Glut 1 glucose transporter isoform. The enhancement of glucose transport in Clone 9 cells in response to H₂O₂was significantly attenuated by genistein and the phospholipase C (PLC) inhibitor, U73122. Exposure to H₂O₂resulted in a rise in cellsn-1,2-diacylglycerol content, and the rise was significantly inhibited by U73122. Moreover, the H₂O₂-induced stimulation of glucose transport was significantly blocked by thapsigargin. Neither staurosporine nor a 24-h preincubation in the presence of phorbol-12-myristate-13-acetate (TPA) affected the stimulatory effect of hydrogen peroxide on glucose transport. The activity of big mitogen-activated kinase (BMK1) and of stress-activated protein kinase (SAPK), both members of mitogen-activated protein kinases, were enhanced in response to exposure to H₂O₂; however, neither protein kinase appeared to be linked to the enhancement of glucose transport by H₂O₂. It is concluded that the stimulation of glucose transport in response to H₂O₂is independent of changes in PKC, BMK1, and SAPK activity, and is mediated, at least in part, through H₂O₂-induced stimulation of protein tyrosine kinase and PLC pathways.

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Regular ArticleMechanism of Stimulation of Glucose Transport by H2O2: Role of Phospholipase C

Abstract

Free Radic. Biol. Med.

Biochim. Biophys. Acta.

FASEB J.

J. Biol. Chem.

J. Biol. Chem.

Blood

Biochem. Biophys. Res. Commun.

Eur. J. Pharmacol.

Cell Biol. Int.

J. Biol. Chem.

Cell Signal.

J. Biol Chem.

FEBS Lett.

J. Biol. Chem.

J. Biol. Chem.

J. Biol. Chem.

J. Biol. Chem.

Diabetes

Annu. Rev. Physiol.

J. Membr. Biol.

Science

Proc. Natl. Acad. Sci. USA

Circ. Res.

Indian J. Biochem. Biophys.

Regular Article
Mechanism of Stimulation of Glucose Transport by H₂O₂: Role of Phospholipase C