Biochemical and Biophysical Research Communications
Regular ArticleCaveolin and MAL, Two Protein Components of Internal Detergent-Insoluble Membranes, Are in Distinct Lipid Microenvironments in MDCK Cells☆
References (22)
- et al.
Cell
(1992) - et al.
Cell
(1990) - et al.
Cell
(1992) - et al.
J. Biol. Chem.
(1996) - et al.
FEBS Lett.
(1995) - et al.
J. Biol. Chem.
(1994) - et al.
Anal. Biochem.
(1987) Curr. Opin. Cell Biol.
(1996)- et al.
Science
(1989) - et al.
J. Cell Biol.
(1989)
Cited by (40)
Epsilon toxin from Clostridium perfringens induces cytotoxicity in FRT thyroid epithelial cells
2018, AnaerobeCitation Excerpt :MAL protein is expressed in FRT and in MDCK cells [26–29]. In addition, in MDCK cells, caveolin and MAL are two protein components of internal DRMs, although in distinct lipid microenvironments [39]. Here, we have described a new cell line of thyroid origin that does not express caveolin 1 but is sensitive to Etx and could be a useful model to study MAL-Etx interaction.
Productive entry of type C foot-and-mouth disease virus into susceptible cultured cells requires clathrin and is dependent on the presence of plasma membrane cholesterol
2007, VirologyCitation Excerpt :The rabbit polyclonal antibody anti-caveolin 1 used showed that both cell lines expressed caveolin-1, being the level of expression detected higher in BHK-21 cells. As reported (Millan et al., 1997), no caveolin-1 expression was detected in Jurkat cells, used as control. Fig. 6B shows the immunofluorescence pattern of caveolin-1 in IBRS-2, which appeared as membrane-associated vesicles, corresponding to lipid-rafts and caveolae, as well as with a perinuclear distribution characteristic of a Golgi location, as reported (Kurzchalia et al., 1994).
Fas ligand is localized to membrane rafts, where it displays increased cell death-inducing activity
2006, BloodCitation Excerpt :Despite the copurification of caveolin by Lalor et al,56 it remains likely that rafts had in fact been isolated together with the caveolae. Our own conclusion that FasL is localized to rafts rather than to caveolae (at least in those cells analyzed by us) is based on the fact that Jurkat cells, in which we detected FasL in detergent-resistant membranes, do not contain caveolae.57 It is unknown which of the FasL properties that change to rafts on recruitment is important for its increased killing capacity.
Identification of genes involved in resistance to interferon-α in cutaneous T-cell lymphoma
2002, American Journal of PathologyCitation Excerpt :CAV1, a caveolin underexpressed by IFN-resistant cells, is also a membrane raft protein. However MAL and CAV1 are found in distinct membrane microdomains/rafts,29 thus suggesting that they may have specialized functions. CAV1 is a tumor suppressor gene, localized on chromosome 7q31, for which a role as a negative regulator of the Ras-p42/44 MAP kinase cascade has been proposed (Figure 7).
MAL Mediates Apical Transport of Secretory Proteins in Polarized Epithelial Madin-Darby Canine Kidney Cells
2001, Journal of Biological ChemistryCitation Excerpt :Cholesterol sequestration with CD inhibits secretion of Tg in MDCK cells, implicating lipid rafts in Tg transport (27). The same treatment did not affect the residence of MAL in rafts, probably because of its strong association with those specialized membranes as revealed by its resistance to solubilization even at a high concentration of detergent (36). In contrast, incorporation of newly synthesized Tg into rafts was greatly diminished by CD treatment, suggesting a weak association of the protein with the rafts.
Cell biology of caveolae and caveolin
2001, Advanced Drug Delivery Reviews
- ☆
Abbreviations used: GPI, glycosylphosphatidylinositolMDCK, Madin-Darby canine kidney;
- 1
To whom correspondence should be addressed. Fax: 34-1-397 8087. E-mail: [email protected].