Elsevier

Experimental Neurology

Volume 166, Issue 1, November 2000, Pages 83-89
Experimental Neurology

Regular Article
Onion-Bulb Formation after a Single Compression Injury in the Macrophage Scavenger Receptor Knockout Mice

https://doi.org/10.1006/exnr.2000.7495Get rights and content

Abstract

Onion-bulb (OB) formation is often encountered in acquired neuropathies such as chronic inflammatory demyelinating polyradiculoneuropathy and diabetic neuropathy and is believed to require repeated injuries to peripheral nerves. Although this suggests that remaining damaged cell membranes, including myelin debris, might trigger OB formation, the molecular mechanism remains unclear. In this study, we were successful in producing many small OBs after a single compression injury to peripheral nerves of the knockout mice deficient of macrophage scavenger receptor class A (MSR-A). Although morphometry showed no difference in the average densities of the remaining myelinating fibers between wild-type and MSR-A knockout mice after the compression injury, there were more macrophages and myelin debris positive for oxidized-phosphatidylcholine in the nerves from the MSR-A knockout mice. We believe that OB formation was induced after a single compression injury as the result of delayed phagocytosis of myelin debris possessing oxidized lipids by MSR-A deficient macrophages. The present work shed light on the molecular mechanism of OB formation seen in chronic neuropathies and provided a model for further investigation.

References (36)

  • A.A.W.M. Gabreëls-Festen et al.

    Charcot-Marie-Tooth disease type 1A: Morphological phenotype of the 17p duplication versus PMP22 point mutations

    Acta Neuropathol.

    (1995)
  • J.F. Goodrum et al.

    Fate of myelin lipids during degeneration and regeneration of peripheral nerve: An autoradiographic study

    J. Neurosci.

    (1994)
  • J.F. Goodrum et al.

    The cell biology of myelin degeneration and regeneration in the peripheral nervous system

    J. Neuropathol. Exp. Neurol.

    (1996)
  • J.W. Griffin et al.

    Macrophage systems in peripheral nerves. A review

    J. Neuropathol. Exp. Neurol.

    (1993)
  • M.J. Ignatius et al.

    Expression of apolipoprotein E during nerve degeneration and regeneration

    Proc. Natl. Acad. Sci. USA

    (1986)
  • M.J. Ignatius et al.

    Lipoprotein uptake by neuronal growth cones in vitro

    Science

    (1987)
  • D.A. Krendel et al.

    Sural nerve biopsy in chronic inflammatory demyelinating polyradiculoneuropathy

    Muscle Nerve

    (1989)
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